DNA methylation signature in blood mirrors successful weight-loss during lifestyle interventions: the CENTRAL trial

  • Maria Keller (Creator)
  • Anat Yaskolka Meir (Creator)
  • Stephan H. Bernhart (Creator)
  • Yftach Gepner (Creator)
  • Ilan Shelef (Creator)
  • Dan Schwarzfuchs (Creator)
  • Gal Tsaban (Creator)
  • Hila Zelicha (Creator)
  • Lydia Hopp (Creator)
  • Luise Müller (Creator)
  • Kerstin Rohde (Creator)
  • Yvonne Böttcher (Creator)
  • Peter F. Stadler (Creator)
  • Michael Stumvoll (Creator)
  • Matthias Blüher (Creator)
  • Peter Kovacs (Creator)
  • Iris Shai (Creator)



Abstract Background One of the major challenges in obesity treatment is to explain the high variability in the individual’s response to specific dietary and physical activity interventions. With this study, we tested the hypothesis that specific DNA methylation changes reflect individual responsiveness to lifestyle intervention and may serve as epigenetic predictors for a successful weight-loss. Methods We conducted an explorative genome-wide DNA methylation analysis in blood samples from 120 subjects (90% men, mean ± SD age = 49 ± 9 years, body mass-index (BMI) = 30.2 ± 3.3 kg/m2) from the 18-month CENTRAL randomized controlled trial who underwent either Mediterranean/low-carbohydrate or low-fat diet with or without physical activity. Results Analyses comparing male subjects with the most prominent body weight-loss (responders, mean weight change − 16%) vs. non-responders (+ 2.4%) (N = 10 each) revealed significant variation in DNA methylation of several genes including LRRC27, CRISP2, and SLFN12 (all adj. P
Date made available17 Nov 2020

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