WHONDRS Summer 2019 Sampling Campaign: Global River Corridor Surface Water FTICR-MS, NPOC, TN, Anions, Stable Isotopes, and Bacterial Abundance

  • Jason G Toyoda (Creator)
  • Amy E Goldman (Creator)
  • Shai Arnon (Creator)
  • Edo Bar-Zeev (Creator)
  • Rosalie K Chu (Creator)
  • Robert E Danczak (Creator)
  • Rebecca A Daly (Creator)
  • Brieanne Forbes (Creator)
  • Vanessa A Garayburu-Caruso (Creator)
  • Emily B. Graham (Creator)
  • Xinming Lin (Creator)
  • James J Moran (Creator)
  • Huiying Ren (Creator)
  • Lupita Renteria (Creator)
  • Charles T Resch (Creator)
  • Malak Tfaily (Creator)
  • Nikola Tolic (Creator)
  • Joshua M Torgeson (Creator)
  • Jacqueline Wells (Creator)
  • Kelly C Wrighton (Creator)
  • James C Stegen (Creator)
  • The WHONDRS Consortium (Creator)



The WHONDRS Summer 2019 Sampling (S19S) Campaign collected samples in 97 globally distributed river corridor systems between July and September 2019. Surficial streambed sediments were collected at three locations within each site (upstream, midstream, and downstream). Surface water was collected at the downstream site. This dataset includes a portion of the data types produced from the surface water samples and does not include any results from the sediment. Sediment data can be found at https://data.ess-dive.lbl.gov/view/doi:10.15485/1729719. Future datasets from this study will include hydrologic and microbial data from the surface water and sediment. The S19S campaign was designed with the science community to ask questions associated with links among core/transient metabolomes, microbial metabolism, biogeochemical function, and physical properties of watershed and river corridor systems. This dataset contains (1) high resolution characterization of dissolved organic matter from surface water via 12 Tesla Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) through the Environmental Molecular Sciences Laboratory (EMSL; https://www.pnnl.gov/environmental-molecular-sciences-laboratory); (2) non-purgeable organic carbon (NPOC); (3) stable water isotopes (O and H); (4) specific conductivity (SpC), total nitrogen (TN), and anions; (5) flow cytometry bacterial abundance data; (6) metadata; (7) file-level metadata (flmd); and (8) data dictionary (dd). The FTICR-MS files are .xml, and the data package includes instructions for using Formularity (https://omics.pnl.gov/software/formularity) and an R script to process the data based on the user’s specific needs. All other files are .csv. This data package was updated in July 2021 to add SpC, TN, and anion data and accompanying documentation. This data package was updated in January 2023 to add flow cytometry bacterial data, accompanying documentation, flmd, and dd. Other data files were not altered during the revisions. See change history for detail.Please use the data package’s DOI to cite the data package. We ask that you email us at WHONDRS@pnnl.gov to let us know that you are using the data and acknowledge WHONDRS and the U.S. Department of Energy's Environmental System Science program—which generously provides funding to WHONDRS—in your publications, proposals, presentations, etc. All data are free to be used for any purpose, such as for manuscripts, presentations, and grant proposals. There is no obligation to include WHONDRS members as co-authors.
Date made available2020
PublisherEnvironmental System Science Data Infrastructure for a Virtual Ecosystem; River Corridor and Watershed Biogeochemistry SFA Worldwide Hydrobiogeochemistry Observation Network for Dynamic River Systems (WHONDRS)
Geographical coverageS19S_0083, Sangamon River, Fisher, Illinois, USA, NSF Critical Zone Observatory (CZO) site: Intensively Managed Landscapes

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