Δ9-tetrahydrocannabinol immunochemical studies: Haptens, monoclonal antibodies, and a convenient synthesis of radiolabeled Δ9-tetrahydrocannabinol

Longwu Qi, Noboru Yamamoto, Michael M. Meijler, Laurence J. Altobell, George F. Koob, Peter Wirsching, Kim D. Janda

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Immunopharmacotherapy as an approach to combat drugs of abuse has become an active area of investigation. Marijuana is the most commonly used illicit drug in the U.S. The main active chemical in marijuana is Δ9- tetrahydrocannabinol (Δ9-THC); hence, monoclonal antibodies with high affinity and specificity for Δ9-tetrahydrocannabinol could be valuable immunopharmacotherapeutic intervention and diagnostic tools. We have synthesized immunoconjugates that induce an effective immune response to Δ9-THC and describe a convenient synthesis of radiolabeled Δ9-THC. We demonstrate the value and use of this probe to select anti-Δ9-THC antibodies that bind Δ9-THC with good affinity. The synthetic route to radiolabeled Δ9-THC has enabled the correct assessment of the affinity of these antibodies to their ligand and may facilitate future binding studies between Δ9- THC and its analogues and the cannabinoid receptors.

Original languageEnglish
Pages (from-to)7389-7399
Number of pages11
JournalJournal of Medicinal Chemistry
Volume48
Issue number23
DOIs
StatePublished - 17 Nov 2005
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Medicine
  • Drug Discovery

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