TY - JOUR
T1 - 1,25-Dihydroxyvitamin D-3 alters membrane phospholipid composition and enhances calcium efflux in HL-60 cells
AU - Levy, Rachel
AU - Nathan, Ilana
AU - Shany, Shraga
N1 - Funding Information:
This work was supported in part by a grant of the Israel Cancer Research Fund. The authors wish to thank Mrs. M. Duman for her excellent editorial assistance.
PY - 1987/8/20
Y1 - 1987/8/20
N2 - 1,25-Dihydroxyvitamin D-3 (1,25(OH)2D3) had direct effects on the HL-60 cell membrane. Treatment of HL-60 cells with 1,25(OH)2D3 for short time periods (2-4 hours) caused an increase in calcium efflux. This phenomenon was found to be unrelated to new protein synthesis since it was not inhibited in the presence of RNA and protein synthesis inhibitors. The treatment of the HL-60 cells with 1,25(OH)2D3 for four hours caused changes in their membrane phospholipid composition. The phosphatidylcholine:phosphatidylethanolamine ratio increased from 1.2 to 1.5. Thus the alteration in the phospholipid composition in the membrane induced by 1,25(OH)2D3 may be responsible for the changes in the permeability of the membrane to calcium ions.
AB - 1,25-Dihydroxyvitamin D-3 (1,25(OH)2D3) had direct effects on the HL-60 cell membrane. Treatment of HL-60 cells with 1,25(OH)2D3 for short time periods (2-4 hours) caused an increase in calcium efflux. This phenomenon was found to be unrelated to new protein synthesis since it was not inhibited in the presence of RNA and protein synthesis inhibitors. The treatment of the HL-60 cells with 1,25(OH)2D3 for four hours caused changes in their membrane phospholipid composition. The phosphatidylcholine:phosphatidylethanolamine ratio increased from 1.2 to 1.5. Thus the alteration in the phospholipid composition in the membrane induced by 1,25(OH)2D3 may be responsible for the changes in the permeability of the membrane to calcium ions.
KW - Calcium ion efflux
KW - Dihydroxyvitamin D-3
KW - Phospholipid composition
UR - http://www.scopus.com/inward/record.url?scp=0023615658&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(87)90293-8
DO - 10.1016/0005-2736(87)90293-8
M3 - Article
AN - SCOPUS:0023615658
SN - 0005-2736
VL - 902
SP - 178
EP - 182
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
IS - 2
ER -