1,25-Dihydroxyvitamin D-3 alters membrane phospholipid composition and enhances calcium efflux in HL-60 cells

Rachel Levy, Ilana Nathan, Shraga Shany

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

1,25-Dihydroxyvitamin D-3 (1,25(OH)2D3) had direct effects on the HL-60 cell membrane. Treatment of HL-60 cells with 1,25(OH)2D3 for short time periods (2-4 hours) caused an increase in calcium efflux. This phenomenon was found to be unrelated to new protein synthesis since it was not inhibited in the presence of RNA and protein synthesis inhibitors. The treatment of the HL-60 cells with 1,25(OH)2D3 for four hours caused changes in their membrane phospholipid composition. The phosphatidylcholine:phosphatidylethanolamine ratio increased from 1.2 to 1.5. Thus the alteration in the phospholipid composition in the membrane induced by 1,25(OH)2D3 may be responsible for the changes in the permeability of the membrane to calcium ions.

Original languageEnglish
Pages (from-to)178-182
Number of pages5
JournalBiochimica et Biophysica Acta - Biomembranes
Volume902
Issue number2
DOIs
StatePublished - 20 Aug 1987

Keywords

  • Calcium ion efflux
  • Dihydroxyvitamin D-3
  • Phospholipid composition

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