Abstract
Particles analysis, such as cell counting and differentiation, are widely used for the diagnosis and monitoring of several medical conditions, such as during inflammation. Three-dimensional-printed lab-on-a-chip (LOC) devices, which can utilize one of the cell counting methods, can bring this technology to remote locations through its cost-efficient advantages and easy handling. We present a three-dimensional-printed LOC device with integrated electrodes. To overcome the limited resolution of a 3D printer, we utilized a flow-focusing design. We modeled and simulated the mass transfer and flow dynamics in the LOC by incorporating a flow-focusing design and reached an optimal channel diameter of 0.5 mm, resulting in a flow-focusing distance of <60 µm. We also used electrochemical impedance spectroscopy to enable the dependence of the electrode–solution interface on the flow-focusing properties. Finally, we highlighted the proof-of-concept detection of microspheres (6 µm diameter), which model biological cells that flow in the channel, by recording the electrochemical impedance at 10 kHz, thus showing the potential of a future point-of-care (POC) device.
Original language | English |
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Article number | 283 |
Journal | Chemosensors |
Volume | 11 |
Issue number | 5 |
DOIs | |
State | Published - 1 May 2023 |
Keywords
- 3D printing
- electrochemical impedance spectroscopy
- finite element method modeling
- flow cytometry
- flow focusing
- fused filament fabrication
- lab-on-a-chip
- microfluidics
- point-of-care detection
- rapid prototyping
ASJC Scopus subject areas
- Analytical Chemistry
- Physical and Theoretical Chemistry