3D Spheroid Imaging Enhancement via Point Spread Function Engineering in High-Throughput Microscopy

Noam Zoref; Maytal Avrashami; Nadav Opatovski; Ilana Barzilai; Onit Alalouf; Yosi Shamay; Yoav Shechtman

doi:10.1364/COSI.2025.CM1A.4

3D Spheroid Imaging Enhancement via Point Spread Function Engineering in High-Throughput Microscopy

Noam Zoref
, Maytal Avrashami
, Nadav Opatovski
, Ilana Barzilai
, Onit Alalouf
, Yosi Shamay
, Yoav Shechtman

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

We develop methods for 3D spheroid imaging, utilizing point spread function engineered high-throughput microscopes, and use them to characterize 3D spheroid-fibroblast interactions and improve extended depth-of-field imaging by neural net analysis.

Original language	English
Title of host publication	Computational Optical Sensing and Imaging, COSI 2025 in Proceedings Optica Imaging Congress 2025, 3D, DH, COSI, IS, pcAOP, RadIT - Part of Optica Imaging Congress
Publisher	Optical Society of America
ISBN (Electronic)	9781557529374
DOIs	https://doi.org/10.1364/COSI.2025.CM1A.4
State	Published - 1 Jan 2025
Externally published	Yes
Event	2025 Computational Optical Sensing and Imaging, COSI 2025 - Seattle, United States Duration: 18 Aug 2025 → 21 Aug 2025

Publication series

Name	Computational Optical Sensing and Imaging, COSI 2025 in Proceedings Optica Imaging Congress 2025, 3D, DH, COSI, IS, pcAOP, RadIT - Part of Optica Imaging Congress

Conference

Conference	2025 Computational Optical Sensing and Imaging, COSI 2025
Country/Territory	United States
City	Seattle
Period	18/08/25 → 21/08/25

ASJC Scopus subject areas

General Computer Science
Control and Systems Engineering
Electrical and Electronic Engineering
Electronic, Optical and Magnetic Materials
Instrumentation
Atomic and Molecular Physics, and Optics

Access to Document

10.1364/COSI.2025.CM1A.4

Cite this

Zoref, N., Avrashami, M., Opatovski, N., Barzilai, I., Alalouf, O., Shamay, Y., & Shechtman, Y. (2025). 3D Spheroid Imaging Enhancement via Point Spread Function Engineering in High-Throughput Microscopy. In Computational Optical Sensing and Imaging, COSI 2025 in Proceedings Optica Imaging Congress 2025, 3D, DH, COSI, IS, pcAOP, RadIT - Part of Optica Imaging Congress (Computational Optical Sensing and Imaging, COSI 2025 in Proceedings Optica Imaging Congress 2025, 3D, DH, COSI, IS, pcAOP, RadIT - Part of Optica Imaging Congress). Optical Society of America. https://doi.org/10.1364/COSI.2025.CM1A.4

Zoref, Noam ; Avrashami, Maytal ; Opatovski, Nadav et al. / 3D Spheroid Imaging Enhancement via Point Spread Function Engineering in High-Throughput Microscopy. Computational Optical Sensing and Imaging, COSI 2025 in Proceedings Optica Imaging Congress 2025, 3D, DH, COSI, IS, pcAOP, RadIT - Part of Optica Imaging Congress. Optical Society of America, 2025. (Computational Optical Sensing and Imaging, COSI 2025 in Proceedings Optica Imaging Congress 2025, 3D, DH, COSI, IS, pcAOP, RadIT - Part of Optica Imaging Congress).

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title = "3D Spheroid Imaging Enhancement via Point Spread Function Engineering in High-Throughput Microscopy",

abstract = "We develop methods for 3D spheroid imaging, utilizing point spread function engineered high-throughput microscopes, and use them to characterize 3D spheroid-fibroblast interactions and improve extended depth-of-field imaging by neural net analysis.",

author = "Noam Zoref and Maytal Avrashami and Nadav Opatovski and Ilana Barzilai and Onit Alalouf and Yosi Shamay and Yoav Shechtman",

note = "Publisher Copyright: {\textcopyright} Optica Publishing Group 2025.; 2025 Computational Optical Sensing and Imaging, COSI 2025 ; Conference date: 18-08-2025 Through 21-08-2025",

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day = "1",

doi = "10.1364/COSI.2025.CM1A.4",

language = "English",

series = "Computational Optical Sensing and Imaging, COSI 2025 in Proceedings Optica Imaging Congress 2025, 3D, DH, COSI, IS, pcAOP, RadIT - Part of Optica Imaging Congress",

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Zoref, N, Avrashami, M, Opatovski, N, Barzilai, I, Alalouf, O, Shamay, Y & Shechtman, Y 2025, 3D Spheroid Imaging Enhancement via Point Spread Function Engineering in High-Throughput Microscopy. in Computational Optical Sensing and Imaging, COSI 2025 in Proceedings Optica Imaging Congress 2025, 3D, DH, COSI, IS, pcAOP, RadIT - Part of Optica Imaging Congress. Computational Optical Sensing and Imaging, COSI 2025 in Proceedings Optica Imaging Congress 2025, 3D, DH, COSI, IS, pcAOP, RadIT - Part of Optica Imaging Congress, Optical Society of America, 2025 Computational Optical Sensing and Imaging, COSI 2025, Seattle, United States, 18/08/25. https://doi.org/10.1364/COSI.2025.CM1A.4

3D Spheroid Imaging Enhancement via Point Spread Function Engineering in High-Throughput Microscopy. / Zoref, Noam; Avrashami, Maytal; Opatovski, Nadav et al.
Computational Optical Sensing and Imaging, COSI 2025 in Proceedings Optica Imaging Congress 2025, 3D, DH, COSI, IS, pcAOP, RadIT - Part of Optica Imaging Congress. Optical Society of America, 2025. (Computational Optical Sensing and Imaging, COSI 2025 in Proceedings Optica Imaging Congress 2025, 3D, DH, COSI, IS, pcAOP, RadIT - Part of Optica Imaging Congress).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AU - Avrashami, Maytal

AU - Opatovski, Nadav

AU - Barzilai, Ilana

AU - Alalouf, Onit

AU - Shamay, Yosi

AU - Shechtman, Yoav

PY - 2025/1/1

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M3 - Conference contribution

AN - SCOPUS:105031651067

T3 - Computational Optical Sensing and Imaging, COSI 2025 in Proceedings Optica Imaging Congress 2025, 3D, DH, COSI, IS, pcAOP, RadIT - Part of Optica Imaging Congress

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Zoref N, Avrashami M, Opatovski N, Barzilai I, Alalouf O, Shamay Y et al. 3D Spheroid Imaging Enhancement via Point Spread Function Engineering in High-Throughput Microscopy. In Computational Optical Sensing and Imaging, COSI 2025 in Proceedings Optica Imaging Congress 2025, 3D, DH, COSI, IS, pcAOP, RadIT - Part of Optica Imaging Congress. Optical Society of America. 2025. (Computational Optical Sensing and Imaging, COSI 2025 in Proceedings Optica Imaging Congress 2025, 3D, DH, COSI, IS, pcAOP, RadIT - Part of Optica Imaging Congress). doi: 10.1364/COSI.2025.CM1A.4

3D Spheroid Imaging Enhancement via Point Spread Function Engineering in High-Throughput Microscopy

Abstract

Publication series

Conference

ASJC Scopus subject areas

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