3D structure prediction and molecular dynamics simulation studies of GPR139

Aman Chandra Kaushik, Shakti Sahi

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

Basic characteristic feature of G-protein coupled receptors (GPCRs) is that they are differentially expressed in different cells in the human body. Orphan GPCRs endogenous substrates are unknown but they are reported to be involved as major drug targets in pharmaceuticals. Probable G-protein coupled receptor 139 (GPR139), belonging to class A GPCR, is present in humans and is encoded by GPR139 gene. 3D structure prediction of GPR139 was done using threading and abinitio methods. The validation and annotation were carried out for optimized model selection. Molecular dynamics (MD) simulation of GPR139 was performed for 300ns to investigate variability of predicted model as well as seven tans membrane (7TM) domain and active site fluctuation. The active site residues were identified to investigate the potential ligand binding sites for inhibition of protein dimerization and neuropeptide receptor activity. The 3D-structure of GPR139 will be beneficial in virtual screening studies to identify potential lead compounds for therapeutic purpose.

Original languageEnglish
Title of host publicationBSB 2016 - International Conference on Bioinformatics and Systems Biology
PublisherInstitute of Electrical and Electronics Engineers
ISBN (Electronic)9781509022618
DOIs
StatePublished - 24 Aug 2016
Externally publishedYes
Event2016 International Conference on Bioinformatics and Systems Biology, BSB 2016 - Allahabad, India
Duration: 4 Mar 20166 Mar 2016

Publication series

NameBSB 2016 - International Conference on Bioinformatics and Systems Biology

Conference

Conference2016 International Conference on Bioinformatics and Systems Biology, BSB 2016
Country/TerritoryIndia
CityAllahabad
Period4/03/166/03/16

Keywords

  • 7TM
  • GPR139
  • MD Simulation
  • Molecular Modelling

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Biochemistry
  • Biotechnology
  • Cancer Research
  • Genetics

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