A 23187-stimulated calcium uptake and GABA release by cerebrocortical synaptosomes: effects of high pressure

S. C. Gilman; J. S. Colton; Y. Grossman

doi:10.1007/BF01250371

A 23187-stimulated calcium uptake and GABA release by cerebrocortical synaptosomes: effects of high pressure

S. C. Gilman, J. S. Colton, Y. Grossman

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Guinea pig cerebrocortical synaptosome preparations were used to study the effect of compression to 62 ATA on⁴⁵Ca²⁺ uptake and [³H]GABA release using a calcium ionophore A23187, which bypasses the voltage-sensitive calcium channel. Pressure was found to exert a suppressive effect on the A 23187-induced release of [³H]GABA, while having no significant effect on A 23187-stimulated⁴⁵Ca²⁺ uptake. On the other hand, both depolarization-induced⁴⁵Ca²⁺ uptake and [³H]GABA release were inhibited by pressure exposure. These results suggest that pressure may suppress GABA release by affecting pre-synaptic events subsequent to calcium influx.

Original language	English
Pages (from-to)	1-9
Number of pages	9
Journal	Journal of Neural Transmission
Volume	86
Issue number	1
DOIs	https://doi.org/10.1007/BF01250371
State	Published - 1 Feb 1991
Externally published	Yes

Keywords

A23187
GABA release
HPNS
Pressure
ionophore

ASJC Scopus subject areas

Neurology
Clinical Neurology
Psychiatry and Mental health
Biological Psychiatry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/BF01250371

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abstract = "Guinea pig cerebrocortical synaptosome preparations were used to study the effect of compression to 62 ATA on45Ca2+ uptake and [3H]GABA release using a calcium ionophore A23187, which bypasses the voltage-sensitive calcium channel. Pressure was found to exert a suppressive effect on the A 23187-induced release of [3H]GABA, while having no significant effect on A 23187-stimulated45Ca2+ uptake. On the other hand, both depolarization-induced45Ca2+ uptake and [3H]GABA release were inhibited by pressure exposure. These results suggest that pressure may suppress GABA release by affecting pre-synaptic events subsequent to calcium influx.",

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AB - Guinea pig cerebrocortical synaptosome preparations were used to study the effect of compression to 62 ATA on45Ca2+ uptake and [3H]GABA release using a calcium ionophore A23187, which bypasses the voltage-sensitive calcium channel. Pressure was found to exert a suppressive effect on the A 23187-induced release of [3H]GABA, while having no significant effect on A 23187-stimulated45Ca2+ uptake. On the other hand, both depolarization-induced45Ca2+ uptake and [3H]GABA release were inhibited by pressure exposure. These results suggest that pressure may suppress GABA release by affecting pre-synaptic events subsequent to calcium influx.

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A 23187-stimulated calcium uptake and GABA release by cerebrocortical synaptosomes: effects of high pressure

Abstract

Keywords

ASJC Scopus subject areas

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