@article{ff4d55f173c14b419051ac9d83dee518,
title = "A Bacterial Quorum Sensing Molecule Elicits a General Stress Response in Saccharomyces cerevisiae",
abstract = "Bacteria assess their population density through a chemical communication mechanism termed quorum sensing, in order to coordinate group behavior. Most research on quorum sensing has focused primarily on its role as an intraspecies chemical signaling mechanism that enables the regulation of certain phenotypes through targeted gene expression. However, in recent years several seminal studies have revealed important phenomena in which quorum sensing molecules appear to serve additional roles as interspecies signals that may regulate microbial ecology. In this study, we asked whether the budding yeast Saccharomyces cerevisiae can sense chemical signals from prokaryotes. When exposed to a variety of quorum sensing molecules from different bacterial species and from Candida albicans we found that N-(3-oxododecanoyl)-L-homoserine lactone (C12) from the opportunistic human pathogen Pseudomonas aeruginosa induces a remarkable stress response in yeast. Microarray experiments confirmed and aided in interpreting these findings, showing a unique and specific expression pattern that differed significantly from the response to previously described stress factors. We further characterized this response and report preliminary findings on the molecular basis for the recognition of C12 by the yeast.",
keywords = "Msn 2/4, N-acyl homoserine lactones, Pseudomonas aeruginosa, Saccharomyces cerevisiae, interkingdom communication, quorum sensing, stress response",
author = "Antonia Delago and Rachel Gregor and Luba Dubinsky and Rambabu Dandela and Adi Hendler and Pnina Krief and Josep Rayo and Amir Aharoni and Meijler, {Michael M.}",
note = "Funding Information: Microarray analysis was performed with the help of Dr. Micha Volokita at the DNA Microarray and Sequencing Unit of the National Institute for Biotechnology in the Negev (NIBN, Ben Gurion University of the Negev). Bioinformatic data analysis of microarray data was performed with the help of Drs. Vered Caspi and Inbar Plaschke from our on-campus Bioinformatics Core Facility. Mass spectrometry (Orbitrap) analyses were performed with the help of the Smoler Proteomics Center (The Technion, Haifa, Israel). We want to sincerely thank Prof. Pamela Silver at Harvard Medical School (Boston, MA) who provided us with a plasmid for GST-labeled eIF5A, Prof. Maya Schuldiner from the Weizmann Institute of Science (Israel) for helpful discussions and for providing us with S. cerevisiae eIF5A mutant strains, and Prof. Jonathan Weissman at the University of California, San Francisco for providing us with S. cerevisiae BY4741. We further want to thank our colleagues at Ben Gurion University of the Negev, Ilana Berger-Fridman, Dr. Mehtap Abu-Quarn, and Dror Baran, for their technical assistance, and to thank Prof. Pieter Dorrestein (University of California, San Diego) and his group, and Dr. Aur{\'e}lie Deveau (INRA and Universit{\'e} de Lorraine, France) for assistance and helpful conversations regarding this work. RG is grateful to the Azrieli Foundation for the award of an Azrieli fellowship. Funding Information: This work was funded by the Israel Science Foundation (Personal Grant 749/09, MM and AA), the Germany-Israel Project Grant (DIP ME4476/2, MM) and the European Research Council (Starting Grant 240356, MM). Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2021 Delago, Gregor, Dubinsky, Dandela, Hendler, Krief, Rayo, Aharoni and Meijler.",
year = "2021",
month = sep,
day = "16",
doi = "10.3389/fmicb.2021.632658",
language = "English",
volume = "12",
journal = "Frontiers in Microbiology",
issn = "1664-302X",
publisher = "Frontiers Media S.A.",
}