A bioenergetic profile of non-transformed fibroblasts uncovers a link between death-resistance and enhanced spare respiratory capacity

Kristen P. Nickens; Jakob D. Wikstrom; Orian S. Shirihai; Steven R. Patierno; Susan Ceryak

doi:10.1016/j.mito.2013.09.005

A bioenergetic profile of non-transformed fibroblasts uncovers a link between death-resistance and enhanced spare respiratory capacity

Kristen P. Nickens, Jakob D. Wikstrom, Orian S. Shirihai, Steven R. Patierno, Susan Ceryak

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

Apoptosis-resistance and metabolic imbalances are prominent features of cancer cells. We have recently reported on populations of human fibroblasts that exhibit resistance to mitochondrial-mediated apoptosis, acquired as a result of a single genotoxic exposure. The objective of the present study was to investigate the intrinsic bioenergetic profile of the death-resistant cells, as compared to the clonogenic control cells. Therefore, we analyzed the basic bioenergetic parameters including oxygen consumption and extracellular acidification rates, coupling efficiency, and spare respiratory capacity. Our data demonstrate a strong correlation between enhanced spare respiratory capacity and death-resistance, which we postulate to be indicative of the earliest stages of carcinogenesis.

Original language	English
Pages (from-to)	662-667
Number of pages	6
Journal	Mitochondrion
Volume	13
Issue number	6
DOIs	https://doi.org/10.1016/j.mito.2013.09.005
State	Published - 1 Nov 2013
Externally published	Yes

Keywords

Bioenergetics
Death-resistance
Mitochondria
Spare respiratory capacity

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.mito.2013.09.005

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title = "A bioenergetic profile of non-transformed fibroblasts uncovers a link between death-resistance and enhanced spare respiratory capacity",

abstract = "Apoptosis-resistance and metabolic imbalances are prominent features of cancer cells. We have recently reported on populations of human fibroblasts that exhibit resistance to mitochondrial-mediated apoptosis, acquired as a result of a single genotoxic exposure. The objective of the present study was to investigate the intrinsic bioenergetic profile of the death-resistant cells, as compared to the clonogenic control cells. Therefore, we analyzed the basic bioenergetic parameters including oxygen consumption and extracellular acidification rates, coupling efficiency, and spare respiratory capacity. Our data demonstrate a strong correlation between enhanced spare respiratory capacity and death-resistance, which we postulate to be indicative of the earliest stages of carcinogenesis.",

keywords = "Bioenergetics, Death-resistance, Mitochondria, Spare respiratory capacity",

author = "Nickens, {Kristen P.} and Wikstrom, {Jakob D.} and Shirihai, {Orian S.} and Patierno, {Steven R.} and Susan Ceryak",

note = "Funding Information: The authors would like to acknowledge Dr. Eric Kaldjian for his assistance in this collaborative effort, as well as Dr. Travis O'Brien, Dr. Madhu Lal-Nag, and Dr. Tura Camilli for their helpful discussions and insight. This work was supported by the National Institutes of Health [ R21ES017334 and R01CA107972 to S.C., R01CA107972 supplement to K.N., and R01ES05304 and R01ES09961 to S.P.]. ",

year = "2013",

month = nov,

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doi = "10.1016/j.mito.2013.09.005",

language = "English",

volume = "13",

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journal = "Mitochondrion",

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publisher = "Elsevier B.V.",

number = "6",

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TY - JOUR

T1 - A bioenergetic profile of non-transformed fibroblasts uncovers a link between death-resistance and enhanced spare respiratory capacity

AU - Nickens, Kristen P.

AU - Wikstrom, Jakob D.

AU - Shirihai, Orian S.

AU - Patierno, Steven R.

AU - Ceryak, Susan

N1 - Funding Information: The authors would like to acknowledge Dr. Eric Kaldjian for his assistance in this collaborative effort, as well as Dr. Travis O'Brien, Dr. Madhu Lal-Nag, and Dr. Tura Camilli for their helpful discussions and insight. This work was supported by the National Institutes of Health [ R21ES017334 and R01CA107972 to S.C., R01CA107972 supplement to K.N., and R01ES05304 and R01ES09961 to S.P.].

PY - 2013/11/1

Y1 - 2013/11/1

N2 - Apoptosis-resistance and metabolic imbalances are prominent features of cancer cells. We have recently reported on populations of human fibroblasts that exhibit resistance to mitochondrial-mediated apoptosis, acquired as a result of a single genotoxic exposure. The objective of the present study was to investigate the intrinsic bioenergetic profile of the death-resistant cells, as compared to the clonogenic control cells. Therefore, we analyzed the basic bioenergetic parameters including oxygen consumption and extracellular acidification rates, coupling efficiency, and spare respiratory capacity. Our data demonstrate a strong correlation between enhanced spare respiratory capacity and death-resistance, which we postulate to be indicative of the earliest stages of carcinogenesis.

AB - Apoptosis-resistance and metabolic imbalances are prominent features of cancer cells. We have recently reported on populations of human fibroblasts that exhibit resistance to mitochondrial-mediated apoptosis, acquired as a result of a single genotoxic exposure. The objective of the present study was to investigate the intrinsic bioenergetic profile of the death-resistant cells, as compared to the clonogenic control cells. Therefore, we analyzed the basic bioenergetic parameters including oxygen consumption and extracellular acidification rates, coupling efficiency, and spare respiratory capacity. Our data demonstrate a strong correlation between enhanced spare respiratory capacity and death-resistance, which we postulate to be indicative of the earliest stages of carcinogenesis.

KW - Bioenergetics

KW - Death-resistance

KW - Mitochondria

KW - Spare respiratory capacity

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U2 - 10.1016/j.mito.2013.09.005

DO - 10.1016/j.mito.2013.09.005

M3 - Article

AN - SCOPUS:84885231311

SN - 1567-7249

VL - 13

SP - 662

EP - 667

JO - Mitochondrion

JF - Mitochondrion

IS - 6

ER -

A bioenergetic profile of non-transformed fibroblasts uncovers a link between death-resistance and enhanced spare respiratory capacity

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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