A comparative physical study of two different hydrophilic synthetic latex matrices for the construction of a glucose biosensor

Serge Cosnier, Sabine Szunerits, Robert S. Marks, Andres Novoa, Laurence Puech, Emile Perez, I. Rico-Lattes

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    38 Scopus citations

    Abstract

    Two different biodegradable latex polymers functionalised by hydroxy (1) or gluconamide (2) groups proved to be good immobilisation matrixes for glucose oxidase. The responses of these biosensors to glucose additions were measured by potentiostating the modified electrodes at 0.6 V/SCE in order to oxidise the hydrogen peroxide generated by the enzymatic oxidation of glucose in the presence of oxygen. The response of such electrodes was evaluated as a function of film thickness, pH and temperature. Rotating disk electrode experiments showed the influence of the enzyme on the structure of both latex films, namely a marked improvement in matrix permeability. The high permeability of the latex 1 based enzyme sensor (bilayer, Pm = 8.10 × 10-4 cm s-1) resulted in a high dynamic range. Furthermore, the activation energy for a latex 1 sensor was determined to be 44.55 and 18.03 kJ mol-1, respectively depending on the conformation of the enzyme.

    Original languageEnglish
    Pages (from-to)889-897
    Number of pages9
    JournalTalanta
    Volume55
    Issue number5
    DOIs
    StatePublished - 13 Dec 2001

    Keywords

    • Biosensor
    • Enzyme electrode
    • Glucose oxidase
    • Latex
    • Permeability

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