A droplet-merging platform for comparative functional analysis of m1 and m2 macrophages in response to e. coli-induced stimuli

Evangelia Hondroulis; Alexandru Movila; Pooja Sabhachandani; Saheli Sarkar; Noa Cohen; Toshihisa Kawai; Tania Konry

doi:10.1002/bit.26196

A droplet-merging platform for comparative functional analysis of m1 and m2 macrophages in response to e. coli-induced stimuli

Evangelia Hondroulis, Alexandru Movila, Pooja Sabhachandani, Saheli Sarkar, Noa Cohen, Toshihisa Kawai, Tania Konry

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Microfluidic droplets are used to isolate cell pairs and prevent crosstalk with neighboring cells, while permitting free motility and interaction within the confined space. Dynamic analysis of cellular heterogeneity in droplets has provided insights in various biological processes. Droplet manipulation methods such as fusion and fission make it possible to precisely regulate the localized environment of a cell in a droplet and deliver reagents as required. Droplet fusion strategies achieved by passive mechanisms preserve cell viability and are easier to fabricate and operate. Here, we present a simple and effective method for the co-encapsulation of polarized M1 and M2 macrophages with Escherichia coli (E. coli) by passive merging in an integrated droplet generation, merging, and docking platform. This approach facilitated live cell profiling of effector immune functions in situ and quantitative functional analysis of macrophage heterogeneity. Biotechnol. Bioeng. 2017;114: 705–709.

Original language	English
Pages (from-to)	705-709
Number of pages	5
Journal	Biotechnology and Bioengineering
Volume	114
Issue number	3
DOIs	https://doi.org/10.1002/bit.26196
State	Published - 1 Mar 2017
Externally published	Yes

Keywords

bacteria
droplet merging
M1/M2 macrophages
microfluidic droplets
single cell analysis

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1002/bit.26196

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title = "A droplet-merging platform for comparative functional analysis of m1 and m2 macrophages in response to e. coli-induced stimuli",

abstract = "Microfluidic droplets are used to isolate cell pairs and prevent crosstalk with neighboring cells, while permitting free motility and interaction within the confined space. Dynamic analysis of cellular heterogeneity in droplets has provided insights in various biological processes. Droplet manipulation methods such as fusion and fission make it possible to precisely regulate the localized environment of a cell in a droplet and deliver reagents as required. Droplet fusion strategies achieved by passive mechanisms preserve cell viability and are easier to fabricate and operate. Here, we present a simple and effective method for the co-encapsulation of polarized M1 and M2 macrophages with Escherichia coli (E. coli) by passive merging in an integrated droplet generation, merging, and docking platform. This approach facilitated live cell profiling of effector immune functions in situ and quantitative functional analysis of macrophage heterogeneity. Biotechnol. Bioeng. 2017;114: 705–709.",

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author = "Evangelia Hondroulis and Alexandru Movila and Pooja Sabhachandani and Saheli Sarkar and Noa Cohen and Toshihisa Kawai and Tania Konry",

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AU - Hondroulis, Evangelia

AU - Movila, Alexandru

AU - Sabhachandani, Pooja

AU - Sarkar, Saheli

AU - Cohen, Noa

AU - Kawai, Toshihisa

AU - Konry, Tania

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Microfluidic droplets are used to isolate cell pairs and prevent crosstalk with neighboring cells, while permitting free motility and interaction within the confined space. Dynamic analysis of cellular heterogeneity in droplets has provided insights in various biological processes. Droplet manipulation methods such as fusion and fission make it possible to precisely regulate the localized environment of a cell in a droplet and deliver reagents as required. Droplet fusion strategies achieved by passive mechanisms preserve cell viability and are easier to fabricate and operate. Here, we present a simple and effective method for the co-encapsulation of polarized M1 and M2 macrophages with Escherichia coli (E. coli) by passive merging in an integrated droplet generation, merging, and docking platform. This approach facilitated live cell profiling of effector immune functions in situ and quantitative functional analysis of macrophage heterogeneity. Biotechnol. Bioeng. 2017;114: 705–709.

AB - Microfluidic droplets are used to isolate cell pairs and prevent crosstalk with neighboring cells, while permitting free motility and interaction within the confined space. Dynamic analysis of cellular heterogeneity in droplets has provided insights in various biological processes. Droplet manipulation methods such as fusion and fission make it possible to precisely regulate the localized environment of a cell in a droplet and deliver reagents as required. Droplet fusion strategies achieved by passive mechanisms preserve cell viability and are easier to fabricate and operate. Here, we present a simple and effective method for the co-encapsulation of polarized M1 and M2 macrophages with Escherichia coli (E. coli) by passive merging in an integrated droplet generation, merging, and docking platform. This approach facilitated live cell profiling of effector immune functions in situ and quantitative functional analysis of macrophage heterogeneity. Biotechnol. Bioeng. 2017;114: 705–709.

KW - bacteria

KW - droplet merging

KW - M1/M2 macrophages

KW - microfluidic droplets

KW - single cell analysis

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JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

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ER -

A droplet-merging platform for comparative functional analysis of m1 and m2 macrophages in response to e. coli-induced stimuli

Abstract

Keywords

ASJC Scopus subject areas

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