TY - JOUR
T1 - A fast signal-induced activation of poly(ADP-ribose) polymerase
T2 - A novel downstream target of phospholipase C
AU - Homburg, S.
AU - Visochek, L.
AU - Moran, N.
AU - Dantzer, F.
AU - Priel, E.
AU - Asculai, E.
AU - Schwartz, D.
AU - Rotter, V.
AU - Dekel, N.
AU - Cohen-Armon, M.
PY - 2000/7/24
Y1 - 2000/7/24
N2 - We present the first evidence for a fast activation of the nuclear protein poly(ADP-ribose) polymerase (PARP) by signals evoked in the cell membrane, constituting a novel mode of signaling to the cell nucleus. PARR an abundant, highly conserved, chromatin-bound protein found only in eukaryotes, exclusively catalyzes polyADP-ribosylation of DNA-binding proteins, thereby modulating their activity. Activation of PARR reportedly induced by formation of DNA breaks, is involved in DNA transcription, replication, and repair. Our findings demonstrate an alternative mechanism: a fast activation of PARR evoked by inositol 1,4,5,-trisphosphate-Ca2+ mobilization, that does not involve DNA breaks. These findings identify PARP as a novel downstream target of phospholipase C, and unveil a novel fast signal-induced modification of DNA-binding proteins by polyADP-ribosylation.
AB - We present the first evidence for a fast activation of the nuclear protein poly(ADP-ribose) polymerase (PARP) by signals evoked in the cell membrane, constituting a novel mode of signaling to the cell nucleus. PARR an abundant, highly conserved, chromatin-bound protein found only in eukaryotes, exclusively catalyzes polyADP-ribosylation of DNA-binding proteins, thereby modulating their activity. Activation of PARR reportedly induced by formation of DNA breaks, is involved in DNA transcription, replication, and repair. Our findings demonstrate an alternative mechanism: a fast activation of PARR evoked by inositol 1,4,5,-trisphosphate-Ca2+ mobilization, that does not involve DNA breaks. These findings identify PARP as a novel downstream target of phospholipase C, and unveil a novel fast signal-induced modification of DNA-binding proteins by polyADP-ribosylation.
KW - Brain neurons
KW - Calcium signaling
KW - Electrical stimulation
KW - Inositol 1,4,5- trisphosphate
KW - Poly(ADP-ribose) polymerase
UR - http://www.scopus.com/inward/record.url?scp=0034710285&partnerID=8YFLogxK
U2 - 10.1083/jcb.150.2.293
DO - 10.1083/jcb.150.2.293
M3 - Article
C2 - 10908573
AN - SCOPUS:0034710285
SN - 0021-9525
VL - 150
SP - 293
EP - 307
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 2
ER -
