A general method for the isolation of labelled peptides from affinity-labelled proteins

D. Givol; Y. Weinstein; M. Gorecki; M. Wilchek

doi:10.1016/0006-291X(70)90656-X

A general method for the isolation of labelled peptides from affinity-labelled proteins

D. Givol
, Y. Weinstein
, M. Gorecki
, M. Wilchek

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

A general method for specific isolation of ligand-bound peptides from affinity-labelled proteins is described. The method makes use of the affinity of the native protein to the ligand that was used for labelling of the same protein. Thus the labelled peptide can be isolated in one step in a very rapid procedure. The method was applied successfully to peptides from an antibody and from ribonuclease.

Original language	English
Pages (from-to)	825-830
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	38
Issue number	4
DOIs	https://doi.org/10.1016/0006-291X(70)90656-X
State	Published - 20 Feb 1970
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(70)90656-X

Cite this

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title = "A general method for the isolation of labelled peptides from affinity-labelled proteins",

abstract = "A general method for specific isolation of ligand-bound peptides from affinity-labelled proteins is described. The method makes use of the affinity of the native protein to the ligand that was used for labelling of the same protein. Thus the labelled peptide can be isolated in one step in a very rapid procedure. The method was applied successfully to peptides from an antibody and from ribonuclease.",

author = "D. Givol and Y. Weinstein and M. Gorecki and M. Wilchek",

year = "1970",

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T1 - A general method for the isolation of labelled peptides from affinity-labelled proteins

AU - Givol, D.

AU - Weinstein, Y.

AU - Gorecki, M.

AU - Wilchek, M.

PY - 1970/2/20

Y1 - 1970/2/20

N2 - A general method for specific isolation of ligand-bound peptides from affinity-labelled proteins is described. The method makes use of the affinity of the native protein to the ligand that was used for labelling of the same protein. Thus the labelled peptide can be isolated in one step in a very rapid procedure. The method was applied successfully to peptides from an antibody and from ribonuclease.

AB - A general method for specific isolation of ligand-bound peptides from affinity-labelled proteins is described. The method makes use of the affinity of the native protein to the ligand that was used for labelling of the same protein. Thus the labelled peptide can be isolated in one step in a very rapid procedure. The method was applied successfully to peptides from an antibody and from ribonuclease.

UR - https://www.scopus.com/pages/publications/0014955832

U2 - 10.1016/0006-291X(70)90656-X

DO - 10.1016/0006-291X(70)90656-X

M3 - Article

C2 - 5462703

AN - SCOPUS:0014955832

SN - 0006-291X

VL - 38

SP - 825

EP - 830

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

A general method for the isolation of labelled peptides from affinity-labelled proteins

Abstract

ASJC Scopus subject areas

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