Abstract
An open reading frame potentially encoding a protein of 1995 amino acids (orf1995) has been found in the chloroplast genome of the green alga Chlamydomonas reinhardtii. Besides having a short hydrophobic N-terminal domain with five putative transmembrane helices, the predicted orf1995 product is highly basic. orf1995 might be a homologue of the ycf1 gene in land plants, whose function has not yet been determined. Mutants of C. reinhardtii transformed with a disruption of orf1995 remain heteroplasmic for the wild-type and disrupted alleles of this gene, indicating that the orf1995 product is essential for cell survival.
| Original language | English |
|---|---|
| Pages (from-to) | 649-653 |
| Number of pages | 5 |
| Journal | Molecular and General Genetics |
| Volume | 253 |
| Issue number | 5 |
| DOIs | |
| State | Published - 18 Mar 1997 |
Keywords
- Chlamydomonas reinhardtii
- Chloroplast genome
- Gene activation
- Green algae
- Transmembrane protein
ASJC Scopus subject areas
- Genetics
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