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A MATLAB Pipeline for Spatiotemporal Quantification of Monolayer Cell Migration

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    2 Scopus citations

    Abstract

    In this chapter we present a MATLAB-based computational pipeline for the quantification of monolayer migration assays. Wound healing assay (or scratch assay) is a commonly used in vitro assay to assess collective cell migration. Our pipeline outputs traditional and spatiotemporal readouts that quantify the group migration properties and was previously used for a screen that included thousands of time-lapse sequences. You will learn how to execute the pipeline, the principles behind the design and implementation choices we made, pitfalls, tips, and tricks in using it.

    Original languageEnglish
    Title of host publicationLearning Materials in Biosciences
    PublisherSpringer International Publishing
    Pages175-206
    Number of pages32
    DOIs
    StatePublished - 1 Jan 2022

    Publication series

    NameLearning Materials in Biosciences
    VolumePart F9775
    ISSN (Print)2509-6125
    ISSN (Electronic)2509-6133

    ASJC Scopus subject areas

    • Bioengineering
    • Biochemistry
    • Cell Biology
    • Biomedical Engineering
    • Biochemistry, Genetics and Molecular Biology (miscellaneous)

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