A mouse pancreatic organoid model to compare PD-L1 blocking antibodies

Guangyuan Li, Susmita Ghosh, Ju Me Park, Hyunsu Shin, Mamatha Garige, Gregory Reaman, Carole Sourbier

Research output: Contribution to journalArticlepeer-review

Abstract

Immune checkpoint inhibitors (ICIs) have changed the therapeutic landscape for cancer patients, but diabetes, a rare, severe immune-related endocrinopathy, is linked to ICI therapy. It is unclear whether glycosylation of ICIs may play a role in the development of this adverse event and how the physiological effects of different ICIs on pancreatic cells should be evaluated. We used a mouse pancreatic organoid model to compare three PD-L1 blocking antibodies in the presence or absence of IFNγ using a metabolic bioanalyzer. Modulation of ICI glycosylation altered its metabolic effects on mouse pancreatic organoids, suggesting that this model could be used to monitor and compare ICIs and to study the mechanisms underlying the development of IC-mediated diabetes.

Original languageEnglish
Article number2139886
JournalmAbs
Volume14
Issue number1
DOIs
StatePublished - 1 Jan 2022
Externally publishedYes

Keywords

  • Immune checkpoint inhibitors
  • PD-L1
  • diabetes
  • metabolism
  • pancreatic organoids

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint

Dive into the research topics of 'A mouse pancreatic organoid model to compare PD-L1 blocking antibodies'. Together they form a unique fingerprint.

Cite this