A New Electrolytic Synthesis Method for Few-Layered MoS2 Nanosheets and Their Robust Biointerfacing with Reduced Antibodies

Manil Kukkar, Satish K. Tuteja, Amit L. Sharma, Vinod Kumar, Ashok K. Paul, Ki Hyun Kim, Priyanka Sabherwal, Akash Deep

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

We report an efficient method for the synthesis of few-layered MoS2 nanosheets and demonstrate their application in the label-free detection of the prostate-specific antigen (PSA) cancer marker. As a novel strategy, the electro-dissolution of molybdenum metal sheets in the presence of Na+ and S2- ions led to the formation of Na+ intercalated MoS2. Further exfoliation by ultrasonication yielded the desired formation of few-layered MoS2 nanosheets. After comprehensive characterization, the synthesized MoS2 nanosheets were channeled in a field-effect transistor (FET) microdevice. Chemically reduced anti-PSA antibodies were immobilized on the MoS2 channel above the FET microdevice to construct a specific PSA immunosensor. The antibodies were deliberately reduced to expose the hinge-region disulfide bonds. This approach offered a robust and site-directed immunosensing device through biointerfacing of the sulfhydryl groups (-SH) in the reduced antibody with the surface S atoms of MoS2. This device was validated as an effective immunosensor with a low detection limit (10-5 ng/mL) over a wide linear detection range (10-5 to 75 ng/mL).

Original languageEnglish
Pages (from-to)16555-16563
Number of pages9
JournalACS applied materials & interfaces
Volume8
Issue number26
DOIs
StatePublished - 6 Jul 2016
Externally publishedYes

Keywords

  • FET
  • MoS
  • few-layered nanosheets
  • immunosensor
  • prostate-specific antigen
  • reduced antibody

ASJC Scopus subject areas

  • Materials Science (all)

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