A Novel Agarose Acrobeads Protein A Column for Selective Immunoadsorbance of Whole Blood: Performance, Specificity and Safety

I. Nathan; M. Aharon; G. Reisenfeld; A. Dvilansky

doi:10.3109/10731199209117855

A Novel Agarose Acrobeads Protein A Column for Selective Immunoadsorbance of Whole Blood: Performance, Specificity and Safety

I. Nathan
, M. Aharon
, G. Reisenfeld
, A. Dvilansky

Basic Sciences Divisions

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

The present study describes the performance and safety parameters of a novel column composed of protein A linked to acrobeads. Hemoperfusion of anticoagulated whole human blood was carried out in a closed system. Specific adsorption of IgG was documented in whole blood, plasma and IgG diluted in saline. Specific activity of the column was 3.52 mg/ml beads. These studies revealed slight changes in platelet counts and minor hemolysis. The relatively short period needed for maximal hemoperfusion effect and safety may enable the use of this novel therapeutic approach in clinical practice.

Original language	English
Pages (from-to)	23-30
Number of pages	8
Journal	Biomaterials, Artificial Cells, and Immobilization Biotechnology
Volume	20
Issue number	1
DOIs	https://doi.org/10.3109/10731199209117855
State	Published - 1 Jan 1992

ASJC Scopus subject areas

General Medicine

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AB - The present study describes the performance and safety parameters of a novel column composed of protein A linked to acrobeads. Hemoperfusion of anticoagulated whole human blood was carried out in a closed system. Specific adsorption of IgG was documented in whole blood, plasma and IgG diluted in saline. Specific activity of the column was 3.52 mg/ml beads. These studies revealed slight changes in platelet counts and minor hemolysis. The relatively short period needed for maximal hemoperfusion effect and safety may enable the use of this novel therapeutic approach in clinical practice.

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A Novel Agarose Acrobeads Protein A Column for Selective Immunoadsorbance of Whole Blood: Performance, Specificity and Safety

Abstract

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