A novel immunoassay with direct relevance to protection against organophosphate poisoning

Eliahu Heldman; Ayala Balan; Ora Horowitz; Sarit Ben-Zion; Michael Torten

doi:10.1016/0014-5793(85)81079-6

A novel immunoassay with direct relevance to protection against organophosphate poisoning

Eliahu Heldman, Ayala Balan, Ora Horowitz, Sarit Ben-Zion, Michael Torten

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

Antiparaoxon immune sera were employed in a new immunoassay based on competition between acetylcholinesterase and antibodies for the binding ofparaoxon. Unlike radioimmunoassay, the new assay described herein can be extended to predict the feasibility of antibodies to confer in vivo protection of acetylcholinesterase against organophosphate poisoning. The toxicity of paraoxon was reduced in mice which were pre-injected with the immune sera.

Original language	English
Pages (from-to)	243-248
Number of pages	6
Journal	FEBS Letters
Volume	180
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(85)81079-6
State	Published - 28 Jan 1985
Externally published	Yes

Keywords

Acetylcholinesterase
Antiparaoxon antibody
Immunization
Immunoassay
Organophosphate
Paraoxon

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(85)81079-6

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title = "A novel immunoassay with direct relevance to protection against organophosphate poisoning",

abstract = "Antiparaoxon immune sera were employed in a new immunoassay based on competition between acetylcholinesterase and antibodies for the binding ofparaoxon. Unlike radioimmunoassay, the new assay described herein can be extended to predict the feasibility of antibodies to confer in vivo protection of acetylcholinesterase against organophosphate poisoning. The toxicity of paraoxon was reduced in mice which were pre-injected with the immune sera.",

keywords = "Acetylcholinesterase, Antiparaoxon antibody, Immunization, Immunoassay, Organophosphate, Paraoxon",

author = "Eliahu Heldman and Ayala Balan and Ora Horowitz and Sarit Ben-Zion and Michael Torten",

year = "1985",

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T1 - A novel immunoassay with direct relevance to protection against organophosphate poisoning

AU - Heldman, Eliahu

AU - Balan, Ayala

AU - Horowitz, Ora

AU - Ben-Zion, Sarit

AU - Torten, Michael

PY - 1985/1/28

Y1 - 1985/1/28

N2 - Antiparaoxon immune sera were employed in a new immunoassay based on competition between acetylcholinesterase and antibodies for the binding ofparaoxon. Unlike radioimmunoassay, the new assay described herein can be extended to predict the feasibility of antibodies to confer in vivo protection of acetylcholinesterase against organophosphate poisoning. The toxicity of paraoxon was reduced in mice which were pre-injected with the immune sera.

AB - Antiparaoxon immune sera were employed in a new immunoassay based on competition between acetylcholinesterase and antibodies for the binding ofparaoxon. Unlike radioimmunoassay, the new assay described herein can be extended to predict the feasibility of antibodies to confer in vivo protection of acetylcholinesterase against organophosphate poisoning. The toxicity of paraoxon was reduced in mice which were pre-injected with the immune sera.

KW - Acetylcholinesterase

KW - Antiparaoxon antibody

KW - Immunization

KW - Immunoassay

KW - Organophosphate

KW - Paraoxon

UR - http://www.scopus.com/inward/record.url?scp=0021961485&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(85)81079-6

DO - 10.1016/0014-5793(85)81079-6

M3 - Article

AN - SCOPUS:0021961485

SN - 0014-5793

VL - 180

SP - 243

EP - 248

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

A novel immunoassay with direct relevance to protection against organophosphate poisoning

Abstract

Keywords

ASJC Scopus subject areas

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