@article{d2372dd436a54ebbaed8171bd9c4146c,
title = "A Novel Lensless Miniature Contact Imaging System for Monitoring Calcium Changes in Live Neurons",
abstract = "Here, we report on the design, fabrication, and verification of a novel CMOS-imager-based contact imaging system. We acquired fluorescent images from live neurons by monitoring calcium changes with Fura-2 dye. Our current device consists of a removable absorption filter interfaced with a CMOS imaging sensor and an external DG-4 lamp for excitation. Fura-2 loaded Lymnaea stagnalis neurons were stimulated with dual excitation wavelengths of 340 and 380 nm; our image sensor detected 510-nm emission. We show that our system is capable of detecting intracellular calcium changes in Fura-2 loaded neurons. Further, this sensor also enabled viewing of multiple neurons over a large surface area simultaneously, an option that is not readily available in conventional light microscopy.",
keywords = "Contact Imaging, Fura-2 AM, fluorescence, fluorescent contact imaging",
author = "Mudraboyina, {Anil Kumar} and Lior Blockstein and Luk, {Collin C.} and Syed, {Naweed I.} and Orly Yadid-Pecht",
note = "Funding Information: Manuscript received January 2, 2014; revised January 27, 2014; accepted January 27, 2014. Date of publication February 4, 2014; date of current version February 14, 2014. The work of C. C. Luk was supported by Leaders in Medicine, NSERC, and AI-HS. The work of N. I. Syed was supported by a CIHR grant. The work of L. Blockstein and A. K. Mudraboyina were supported by NSERC. The work of O. Yadid-Pecht was supported by iCORE/AITF. These authors contributed equally to the work presented here. The authors acknowledge M. Agius, Dr. C. Dalton, and Dr. C. Hayden, AMIF Lab, University of Calgary for their help and support in cleanroom with manufacturing of our PVAcB-8 filter. We acknowledge Dr. M. Mintchev for his attention and review of the repeatability tests. We acknowledge Dr. R. J. Turner for access to the spectrometer equipment funded by NSERC, CIHR, and AHFMR. We thank F. Hickley for his contribution and assistance in technical issues. We thank Dr. B. Maundy, Dr. V. Birss, and C. Simon for their insight, suggestions and revision of this work. We wish to express our gratitude to W. Zaidi for his excellent technical contributions to this study. Publisher Copyright: {\textcopyright} 2009-2012 IEEE.",
year = "2014",
month = feb,
day = "1",
doi = "10.1109/JPHOT.2014.2304554",
language = "English",
volume = "6",
journal = "IEEE Photonics Journal",
issn = "1943-0655",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
number = "1",
}