A Novel Lensless Miniature Contact Imaging System for Monitoring Calcium Changes in Live Neurons

Anil Kumar Mudraboyina, Lior Blockstein, Collin C. Luk, Naweed I. Syed, Orly Yadid-Pecht

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Here, we report on the design, fabrication, and verification of a novel CMOS-imager-based contact imaging system. We acquired fluorescent images from live neurons by monitoring calcium changes with Fura-2 dye. Our current device consists of a removable absorption filter interfaced with a CMOS imaging sensor and an external DG-4 lamp for excitation. Fura-2 loaded Lymnaea stagnalis neurons were stimulated with dual excitation wavelengths of 340 and 380 nm; our image sensor detected 510-nm emission. We show that our system is capable of detecting intracellular calcium changes in Fura-2 loaded neurons. Further, this sensor also enabled viewing of multiple neurons over a large surface area simultaneously, an option that is not readily available in conventional light microscopy.

Original languageEnglish
Article number3900115
JournalIEEE Photonics Journal
Volume6
Issue number1
DOIs
StatePublished - 1 Feb 2014
Externally publishedYes

Keywords

  • Contact Imaging
  • Fura-2 AM
  • fluorescence
  • fluorescent contact imaging

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electrical and Electronic Engineering

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