A novel light-harvesting ZIF-9-TCPP as a promising FRET-based ratiometric fluorescence probe for sperm mobility

Yi Xuan Li, Yu Xuan Dai, Ju Zheng Wang, Jérome Chauvin, Xue Ji Zhang, Serge Cosnier, Robert S. Marks, Dan Shan

Research output: Contribution to journalArticlepeer-review

Abstract

The concentration of zinc ions in semen is significantly correlated with sperm viability and male fertility. In this work, a reliable ratiometric fluorescence probe (ZIF-9-TCPP) based on the efficient Förster resonance energy transfer (FRET) process between two luminophores, benzimidazole (BIM) and meso-tetra (4-carboxyphenyl) porphyrin (TCPP) for Zn2+ detection has been constructed, where the emissions of BIM and TCPP are used as reference and detection signals. The proximity of BIM and TCPP in one framework (ZIF-9-TCPP) and the overlapped spectra between BIM and TCPP afford the attainment of a highly efficient FRET (around 90% efficiency). Efficient FRET improves the fluorescence intensity of porphyrin to enhance the sensitivity of detection. The unique spectral shift resulting from Zn2+ binding to the porphyrin ring ensures the selectivity of detection. In addition, the response mechanism of the proposed ratiometric probes to Zn2+ has been investigated. This work provides a convenient way to design an efficient FRET system and a promising method for sperm mobility detection.

Original languageEnglish
Pages (from-to)5784-5790
Number of pages7
JournalAnalyst
Volume149
Issue number24
DOIs
StatePublished - 11 Nov 2024

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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