A phenotypic and functional characterization of NK cells in adenoids

Sa'ar Mizrahi, Eitan Yefenof, Menahem Gross, Pierre Attal, Avraham Ben Yaakov, Debra Goldman-Wohl, Bella Maly, Noam Stern, Gil Katz, Roi Gazit, Ronit Vogt Sionov, Ofer Mandelboim, Stella Chaushu

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Adenoids are part of the MALT. In the present study, we analyzed cell surface markers and cytolytic activity of adenoidal NK (A-NK) cells and compared them with NK cells derived from blood of the same donors (B-NK). NK cells comprised 0.67% (0.4-1.2%) of the total lymphoid population isolated from adenoids. The majority (median=92%) of the A-NK cells was CD56 brightCD16-. A-NK cells were characterized by the increased expression of activation-induced receptors. NKp44 was detected on >60%, CD25 on >40%, and HLA-DR on >50% of freshly isolated A-NK cells. Functional assays indicated that the cytotoxic machinery of A-NK is intact, and sensitive target cells are killed via natural cytotoxicity receptors, such as NKG2D. Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1; CD66) expression was up-regulated in 23% (median) of the A-NK cells by IL-2 activation but unchanged in B-NK cells. CEACAM1 inhibited the A-NK killing of target cells. CXCR4 was expressed on more than 40% A-NK cells prior to activation. Its ligand, CXCL12, was found in endothelial cells of the capillaries within the adenoid and in cells of the epithelial lining. In addition, A-NK cells migrated in vitro toward a gradient of CXCL12 in a dose-responsive manner, suggesting a role for this chemokine in A-NK cell recruitment and trafficking. We conclude that the A-NK cells are unique in that they display an activated-like phenotype and are different from their CD16-B-NK cell counterparts. This phenotype presumably reflects the chronic interaction of A-NK cells with antigens penetrating the body through the nasal route.

Original languageEnglish
Pages (from-to)1095-1105
Number of pages11
JournalJournal of Leukocyte Biology
Volume82
Issue number5
DOIs
StatePublished - 1 Nov 2007
Externally publishedYes

Keywords

  • Chemokines
  • Human
  • Receptors

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