A photoactivable probe for calcium binding proteins

Adrian Israelson, Laetitia Arzoine, Salah Abu-Hamad, Vladimir Khodorkovsky, Varda Shoshan-Barmatz

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Ca2+ as a signaling molecule carries information pivotal to cell life and death via its reversible interaction with a specific site in a protein. Although numerous Ca2+-dependent activities are known, the proteins responsible for some of these activities remain unidentified. We synthesized and characterized a photo-reactive reagent, azido ruthenium (AzRu), which interacts specifically with Ca2+ binding proteins and strongly inhibits their Ca2+-dependent activities, regardless of their catalytic mechanisms or functional state as purified proteins, embedded in the membrane or in intact cells. As expected from a Ca2+ binding protein-specific reagent, AzRu had no effect on Ca2+-independent and Mg2+-dependent activities. Az103Ru covalently bound, and specifically labeled, known Ca2+ binding proteins. AzRu is a photoreactive reagent that provides an approach for identification of Ca 2+ binding proteins, characterization of their binding sites, and exploration of new Ca2+-dependent processes.

Original languageEnglish
Pages (from-to)1169-1178
Number of pages10
JournalChemistry and Biology
Volume12
Issue number11
DOIs
StatePublished - 1 Nov 2005

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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