TY - JOUR
T1 - A polycatenated DNA scaffold for the one-step assembly of hierarchical nanostructures
AU - Weizmann, Yossi
AU - Braunschweig, Adam B.
AU - Wilner, Ofer I.
AU - Cheglakov, Zoya
AU - Willner, Itamar
PY - 2008/4/8
Y1 - 2008/4/8
N2 - A unique DNA scaffold was prepared for the one-step self-assembly of hierarchical nanostructures onto which multiple proteins or nanoparticles are positioned on a single template with precise relative spatial orientation. The architecture is a topologically complex ladder-shaped polycatenane in which the "rungs" of the ladder are used to bring together the individual rings of the mechanically interlocked structure, and the "rails" are available for hierarchical assembly, whose effectiveness has been demonstrated with proteins, complementary DNA, and gold nanoparticles. The ability of this template to form from linear monomers and simultaneously bind two proteins was demonstrated by chemical force microscopy, transmission electron microscopy, and confocal fluorescence microscopy. Finally, fluorescence resonance energy transfer between adjacent fluorophores confirmed the programmed spatial arrangement between two different nanomaterials. DNA templates that bring together multiple nanostructures with precise spatial control have applications in catalysis, biosensing, and nanomaterials design.
AB - A unique DNA scaffold was prepared for the one-step self-assembly of hierarchical nanostructures onto which multiple proteins or nanoparticles are positioned on a single template with precise relative spatial orientation. The architecture is a topologically complex ladder-shaped polycatenane in which the "rungs" of the ladder are used to bring together the individual rings of the mechanically interlocked structure, and the "rails" are available for hierarchical assembly, whose effectiveness has been demonstrated with proteins, complementary DNA, and gold nanoparticles. The ability of this template to form from linear monomers and simultaneously bind two proteins was demonstrated by chemical force microscopy, transmission electron microscopy, and confocal fluorescence microscopy. Finally, fluorescence resonance energy transfer between adjacent fluorophores confirmed the programmed spatial arrangement between two different nanomaterials. DNA templates that bring together multiple nanostructures with precise spatial control have applications in catalysis, biosensing, and nanomaterials design.
KW - Catenane
KW - Chemical force microscopy
KW - Nanoparticle
KW - Proteins
KW - Wires
UR - http://www.scopus.com/inward/record.url?scp=44449126536&partnerID=8YFLogxK
U2 - 10.1073/pnas.0800723105
DO - 10.1073/pnas.0800723105
M3 - Article
C2 - 18391204
AN - SCOPUS:44449126536
SN - 0027-8424
VL - 105
SP - 5289
EP - 5294
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 14
ER -