A polycatenated DNA scaffold for the one-step assembly of hierarchical nanostructures

Yossi Weizmann, Adam B. Braunschweig, Ofer I. Wilner, Zoya Cheglakov, Itamar Willner

Research output: Contribution to journalArticlepeer-review

98 Scopus citations


A unique DNA scaffold was prepared for the one-step self-assembly of hierarchical nanostructures onto which multiple proteins or nanoparticles are positioned on a single template with precise relative spatial orientation. The architecture is a topologically complex ladder-shaped polycatenane in which the "rungs" of the ladder are used to bring together the individual rings of the mechanically interlocked structure, and the "rails" are available for hierarchical assembly, whose effectiveness has been demonstrated with proteins, complementary DNA, and gold nanoparticles. The ability of this template to form from linear monomers and simultaneously bind two proteins was demonstrated by chemical force microscopy, transmission electron microscopy, and confocal fluorescence microscopy. Finally, fluorescence resonance energy transfer between adjacent fluorophores confirmed the programmed spatial arrangement between two different nanomaterials. DNA templates that bring together multiple nanostructures with precise spatial control have applications in catalysis, biosensing, and nanomaterials design.

Original languageEnglish
Pages (from-to)5289-5294
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number14
StatePublished - 8 Apr 2008
Externally publishedYes


  • Catenane
  • Chemical force microscopy
  • Nanoparticle
  • Proteins
  • Wires

ASJC Scopus subject areas

  • General


Dive into the research topics of 'A polycatenated DNA scaffold for the one-step assembly of hierarchical nanostructures'. Together they form a unique fingerprint.

Cite this