A polypyrrole cDNA electrode for the amperometric detection of the West Nile Virus

Rodica E. Ionescu; Sebastien Herrmann; Serge Cosnier; Robert S. Marks

doi:10.1016/j.elecom.2006.08.009

A polypyrrole cDNA electrode for the amperometric detection of the West Nile Virus

Rodica E. Ionescu, Sebastien Herrmann, Serge Cosnier, Robert S. Marks

Department of Biotechnology Engineering

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

The determination of a short cDNA sequence from West Nile Virus (WNV) was carried out with an amperometric DNA sensor. The latter was constituted by an amino-21-mer oligonucleotide probe covalently anchored to a poly(pyrrole-NHS), previously electro-polymerized on a platinum electrode. After incubation with a target model of the WNV cDNA, the hybridization events on the sensor surface were detected by an additional hybridization process with a complementary biotinylated 15-mer WNV cDNA followed by the specific attachment of a biotinylated glucose oxidase via an avidin bridge. The hybridization event was then monitored at 0.6 V vs Ag/AgCl by amperometric detection of H₂O₂, generated by the enzyme marker in the presence of glucose. A relatively short (2 h) hybridization period allows the convenient quantification of the WNV DNA target in the range 10^-10-10^-15 g ml^-1.

Original language	English
Pages (from-to)	1741-1748
Number of pages	8
Journal	Electrochemistry Communications
Volume	8
Issue number	11
DOIs	https://doi.org/10.1016/j.elecom.2006.08.009
State	Published - 1 Nov 2006

Keywords

DNA sensor
Polypyrrole
Pyrrole-NHS
West Nile Virus

ASJC Scopus subject areas

Electrochemistry

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10.1016/j.elecom.2006.08.009

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@article{9eef583f4ce4491c8974c5bb1dec3f14,

title = "A polypyrrole cDNA electrode for the amperometric detection of the West Nile Virus",

abstract = "The determination of a short cDNA sequence from West Nile Virus (WNV) was carried out with an amperometric DNA sensor. The latter was constituted by an amino-21-mer oligonucleotide probe covalently anchored to a poly(pyrrole-NHS), previously electro-polymerized on a platinum electrode. After incubation with a target model of the WNV cDNA, the hybridization events on the sensor surface were detected by an additional hybridization process with a complementary biotinylated 15-mer WNV cDNA followed by the specific attachment of a biotinylated glucose oxidase via an avidin bridge. The hybridization event was then monitored at 0.6 V vs Ag/AgCl by amperometric detection of H2O2, generated by the enzyme marker in the presence of glucose. A relatively short (2 h) hybridization period allows the convenient quantification of the WNV DNA target in the range 10-10-10-15 g ml-1.",

keywords = "DNA sensor, Polypyrrole, Pyrrole-NHS, West Nile Virus",

author = "Ionescu, {Rodica E.} and Sebastien Herrmann and Serge Cosnier and Marks, {Robert S.}",

note = "Funding Information: The authors thank Dr. Naoufel Haddour for his valuable assistance in the synthesis of pyrrole-NHS molecule and Elad Sintov for his support on designing the DNA sequences. Rodica Ionescu thanks the French Ministry of Affairs for supporting her stay at Dr. Serge Cosnier laboratory through a Postdoctoral Chateaubriand Fellowship. The authors also thank the ACI CAPALEX n° 49 “Nouvelles methodologies analytiques et capteurs 2003” for partial financial support. ",

year = "2006",

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doi = "10.1016/j.elecom.2006.08.009",

language = "English",

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journal = "Electrochemistry Communications",

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AU - Ionescu, Rodica E.

AU - Herrmann, Sebastien

AU - Cosnier, Serge

AU - Marks, Robert S.

N1 - Funding Information: The authors thank Dr. Naoufel Haddour for his valuable assistance in the synthesis of pyrrole-NHS molecule and Elad Sintov for his support on designing the DNA sequences. Rodica Ionescu thanks the French Ministry of Affairs for supporting her stay at Dr. Serge Cosnier laboratory through a Postdoctoral Chateaubriand Fellowship. The authors also thank the ACI CAPALEX n° 49 “Nouvelles methodologies analytiques et capteurs 2003” for partial financial support.

PY - 2006/11/1

Y1 - 2006/11/1

N2 - The determination of a short cDNA sequence from West Nile Virus (WNV) was carried out with an amperometric DNA sensor. The latter was constituted by an amino-21-mer oligonucleotide probe covalently anchored to a poly(pyrrole-NHS), previously electro-polymerized on a platinum electrode. After incubation with a target model of the WNV cDNA, the hybridization events on the sensor surface were detected by an additional hybridization process with a complementary biotinylated 15-mer WNV cDNA followed by the specific attachment of a biotinylated glucose oxidase via an avidin bridge. The hybridization event was then monitored at 0.6 V vs Ag/AgCl by amperometric detection of H2O2, generated by the enzyme marker in the presence of glucose. A relatively short (2 h) hybridization period allows the convenient quantification of the WNV DNA target in the range 10-10-10-15 g ml-1.

AB - The determination of a short cDNA sequence from West Nile Virus (WNV) was carried out with an amperometric DNA sensor. The latter was constituted by an amino-21-mer oligonucleotide probe covalently anchored to a poly(pyrrole-NHS), previously electro-polymerized on a platinum electrode. After incubation with a target model of the WNV cDNA, the hybridization events on the sensor surface were detected by an additional hybridization process with a complementary biotinylated 15-mer WNV cDNA followed by the specific attachment of a biotinylated glucose oxidase via an avidin bridge. The hybridization event was then monitored at 0.6 V vs Ag/AgCl by amperometric detection of H2O2, generated by the enzyme marker in the presence of glucose. A relatively short (2 h) hybridization period allows the convenient quantification of the WNV DNA target in the range 10-10-10-15 g ml-1.

KW - DNA sensor

KW - Polypyrrole

KW - Pyrrole-NHS

KW - West Nile Virus

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DO - 10.1016/j.elecom.2006.08.009

M3 - Article

AN - SCOPUS:33751027957

SN - 1388-2481

VL - 8

SP - 1741

EP - 1748

JO - Electrochemistry Communications

JF - Electrochemistry Communications

IS - 11

ER -

A polypyrrole cDNA electrode for the amperometric detection of the West Nile Virus

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Keywords

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