A polypyrrole cDNA electrode for the amperometric detection of the West Nile Virus

Rodica E. Ionescu, Sebastien Herrmann, Serge Cosnier, Robert S. Marks

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

The determination of a short cDNA sequence from West Nile Virus (WNV) was carried out with an amperometric DNA sensor. The latter was constituted by an amino-21-mer oligonucleotide probe covalently anchored to a poly(pyrrole-NHS), previously electro-polymerized on a platinum electrode. After incubation with a target model of the WNV cDNA, the hybridization events on the sensor surface were detected by an additional hybridization process with a complementary biotinylated 15-mer WNV cDNA followed by the specific attachment of a biotinylated glucose oxidase via an avidin bridge. The hybridization event was then monitored at 0.6 V vs Ag/AgCl by amperometric detection of H2O2, generated by the enzyme marker in the presence of glucose. A relatively short (2 h) hybridization period allows the convenient quantification of the WNV DNA target in the range 10-10-10-15 g ml-1.

Original languageEnglish
Pages (from-to)1741-1748
Number of pages8
JournalElectrochemistry Communications
Volume8
Issue number11
DOIs
StatePublished - 1 Nov 2006

Keywords

  • DNA sensor
  • Polypyrrole
  • Pyrrole-NHS
  • West Nile Virus

ASJC Scopus subject areas

  • Electrochemistry

Fingerprint

Dive into the research topics of 'A polypyrrole cDNA electrode for the amperometric detection of the West Nile Virus'. Together they form a unique fingerprint.

Cite this