A presynaptic locus for long-term potentiation of elementary synaptic transmission at mossy fiber synapses in culture

Juan Carlos López-García, Ottavio Arancio, Eric R. Kandel, Danny Baranes

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

The complex circuitry of the CA3 region and the abundance of collateral connections has made it difficult to study the mossy fiber pathway in hippocampal slices and therefore to establish the site of expression of long- term potentiation at these synapses. Using a novel cell culture system, we have produced long-term potentiation of the elementary synaptic connections on single CA3 pyramidal neurons following tetanic stimulation of individual dentate gyrus granule cells. As is the case for the hippocampal slice, this potentiation was independent of N-methyl-D-aspartate receptor activation, was simulated by application of forskolin, and its induction did not require any modulatory input. The increase in synaptic strength was accompanied by a reduction in the number of failures of transmission and by an increase in the coefficient of variation of the responses and was prevented by presynaptic injection of an inhibitor of protein kinase A. These findings show that mossy fiber long-term potentiation has a presynaptic locus and that its expression is dependent on protein kinase A.

Original languageEnglish
Pages (from-to)4712-4717
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number10
DOIs
StatePublished - 14 May 1996
Externally publishedYes

Keywords

  • CA3 region
  • dentate gyrus
  • hippocampus
  • plasticity

ASJC Scopus subject areas

  • General

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