A rapid and easy procedure of biosensor fabrication by micro-encapsulation of enzyme in hydrophilic synthetic latex films. Application to the amperometric determination of glucose

Serge Cosnier, Sabine Szunerits, Robert S. Marks, Andres Novoa, Laurence Puech, Emile Perez, Isabelle Rico-Lattes

    Research output: Contribution to journalArticlepeer-review

    39 Scopus citations

    Abstract

    Novel enzyme electrodes based on synthetic hydrophilic latex matrices are described for the detection of glucose. Glucose oxidase was immobilised through micro-encapsulation, by the simple adsorption of enzyme-latex suspensions on the surface of a platinum electrode. Two latex films functionalised by a hydroxy or a gluconamide group were used. The response of these biosensors to glucose additions was measured by potentiostating the modified electrodes at 0.6 V/SCE in order to oxidise the hydrogen peroxide generated by the enzymatic oxidation of glucose in the presence of dioxygen The response of such electrodes was evaluated as a function of film thickness and temperature. The sensitivity for a two-layer latex-based biosensor was found to be 38.78 mA M-1 cm-1 with a response time of 3-5 s. Moreover, a marked improvement of the thermal stability of the biosensor was observed. Only at temperatures higher than 65°C the enzyme started to be denatured and being inactive.

    Original languageEnglish
    Pages (from-to)851-855
    Number of pages5
    JournalElectrochemistry Communications
    Volume2
    Issue number12
    DOIs
    StatePublished - 1 Dec 2000

    Keywords

    • Biosensor
    • Enzyme electrode
    • Glucose oxidase
    • Latex

    ASJC Scopus subject areas

    • Electrochemistry

    Fingerprint

    Dive into the research topics of 'A rapid and easy procedure of biosensor fabrication by micro-encapsulation of enzyme in hydrophilic synthetic latex films. Application to the amperometric determination of glucose'. Together they form a unique fingerprint.

    Cite this