A simple ELISA capable of distinguishing between IgG antibodies to Chlamydia trachomatis and Chlamydia pneumoniae

Maureen G. Friedman; Shlomo Ilan; Simona Kahane; Nani Kosashvili; Yona Bir; David Lieberman

doi:10.1016/S1386-2618(97)00007-X

A simple ELISA capable of distinguishing between IgG antibodies to Chlamydia trachomatis and Chlamydia pneumoniae

Maureen G. Friedman, Shlomo Ilan, Simona Kahane, Nani Kosashvili, Yona Bir, David Lieberman

Virology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Simple assays which reliably distinguish between past infection with Chlamydia pneumoniae and with Chlamydia trachomatis are needed. We developed an enzyme-linked immunosorbent assay (ELISA) for this purpose by reducing antigen cross reactive lipopolysaccharide epitopes with deoxycholale treatment and releasing antibodies of low affinity with a 6 M urea wash step. Paired serum samples from 212 patients with non-C. pneumoniae-associated community acquired pneumonia and single serum samples from 61 healthy students, 61 women with gynaecological complaints, and 100 blood donors were tested in the assay system. Excellent positive/negative correlation with the microimmunofluorescence (MIF) test was shown. This urea ELISA assay may be useful in assessment of past infection with these organisms, especially in epidemiological studies.

Original language	English
Pages (from-to)	43-49
Number of pages	7
Journal	Opportunistic Pathogens
Volume	9
Issue number	1
DOIs	https://doi.org/10.1016/S1386-2618(97)00007-X
State	Published - 1 Nov 1997

Keywords

Antibody avidity
Chlamydia pneumoniae
Chlamydia serology
Chlamydia trachomatis
Elisa
Seroepidemiology

ASJC Scopus subject areas

Immunology
Microbiology (medical)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/S1386-2618(97)00007-X

Cite this

@article{90be62fcc243481da84c39922aedf5df,

title = "A simple ELISA capable of distinguishing between IgG antibodies to Chlamydia trachomatis and Chlamydia pneumoniae",

abstract = "Simple assays which reliably distinguish between past infection with Chlamydia pneumoniae and with Chlamydia trachomatis are needed. We developed an enzyme-linked immunosorbent assay (ELISA) for this purpose by reducing antigen cross reactive lipopolysaccharide epitopes with deoxycholale treatment and releasing antibodies of low affinity with a 6 M urea wash step. Paired serum samples from 212 patients with non-C. pneumoniae-associated community acquired pneumonia and single serum samples from 61 healthy students, 61 women with gynaecological complaints, and 100 blood donors were tested in the assay system. Excellent positive/negative correlation with the microimmunofluorescence (MIF) test was shown. This urea ELISA assay may be useful in assessment of past infection with these organisms, especially in epidemiological studies.",

keywords = "Antibody avidity, Chlamydia pneumoniae, Chlamydia serology, Chlamydia trachomatis, Elisa, Seroepidemiology",

author = "Friedman, {Maureen G.} and Shlomo Ilan and Simona Kahane and Nani Kosashvili and Yona Bir and David Lieberman",

year = "1997",

month = nov,

day = "1",

doi = "10.1016/S1386-2618(97)00007-X",

language = "English",

volume = "9",

pages = "43--49",

journal = "Opportunistic Pathogens",

issn = "1386-2618",

publisher = "Elsevier BV",

number = "1",

}

TY - JOUR

T1 - A simple ELISA capable of distinguishing between IgG antibodies to Chlamydia trachomatis and Chlamydia pneumoniae

AU - Friedman, Maureen G.

AU - Ilan, Shlomo

AU - Kahane, Simona

AU - Kosashvili, Nani

AU - Bir, Yona

AU - Lieberman, David

PY - 1997/11/1

Y1 - 1997/11/1

N2 - Simple assays which reliably distinguish between past infection with Chlamydia pneumoniae and with Chlamydia trachomatis are needed. We developed an enzyme-linked immunosorbent assay (ELISA) for this purpose by reducing antigen cross reactive lipopolysaccharide epitopes with deoxycholale treatment and releasing antibodies of low affinity with a 6 M urea wash step. Paired serum samples from 212 patients with non-C. pneumoniae-associated community acquired pneumonia and single serum samples from 61 healthy students, 61 women with gynaecological complaints, and 100 blood donors were tested in the assay system. Excellent positive/negative correlation with the microimmunofluorescence (MIF) test was shown. This urea ELISA assay may be useful in assessment of past infection with these organisms, especially in epidemiological studies.

AB - Simple assays which reliably distinguish between past infection with Chlamydia pneumoniae and with Chlamydia trachomatis are needed. We developed an enzyme-linked immunosorbent assay (ELISA) for this purpose by reducing antigen cross reactive lipopolysaccharide epitopes with deoxycholale treatment and releasing antibodies of low affinity with a 6 M urea wash step. Paired serum samples from 212 patients with non-C. pneumoniae-associated community acquired pneumonia and single serum samples from 61 healthy students, 61 women with gynaecological complaints, and 100 blood donors were tested in the assay system. Excellent positive/negative correlation with the microimmunofluorescence (MIF) test was shown. This urea ELISA assay may be useful in assessment of past infection with these organisms, especially in epidemiological studies.

KW - Antibody avidity

KW - Chlamydia pneumoniae

KW - Chlamydia serology

KW - Chlamydia trachomatis

KW - Elisa

KW - Seroepidemiology

UR - http://www.scopus.com/inward/record.url?scp=0031277546&partnerID=8YFLogxK

U2 - 10.1016/S1386-2618(97)00007-X

DO - 10.1016/S1386-2618(97)00007-X

M3 - Article

AN - SCOPUS:0031277546

SN - 1386-2618

VL - 9

SP - 43

EP - 49

JO - Opportunistic Pathogens

JF - Opportunistic Pathogens

IS - 1

ER -

A simple ELISA capable of distinguishing between IgG antibodies to Chlamydia trachomatis and Chlamydia pneumoniae

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this