TY - JOUR
T1 - A single polypeptide catalyzing the conversion of phytoene to ξ-carotene is transcriptionally regulated during tomato fruit ripening
AU - Pecker, Iris
AU - Chamovitz, Daniel
AU - Linden, Hartmut
AU - Sandmann, Gerhard
AU - Hirschberg, Joseph
PY - 1992/1/1
Y1 - 1992/1/1
N2 - The cDNA of the gene pds from tomato, encoding the carotenoid biosynthesis enzyme phytoene desaturase, was cloned, and its nucleotide sequence was determined. Cells of Escherichia coli that expressed the tomato pds gene could convert phytoene to ξ-carotene. This result suggests that one polypeptide, the product of the pds gene, can carry out phytoene desaturation in the carotenoid biosynthetic pathway. Transcripts of the pds gene accumulate in orange tomato fruit, indicating transcriptional control of pds expression during fruit ripening. The deduced amino acid sequence of phytoene desaturase indicates that this enzyme in tomato contains 583 amino acids that are highly conserved with respect to the homologous enzymes in cyanobacteria and algae. The deduced amino acid sequences of the phytoene desaturases from other microorganisms (purple bacteria and fungi) appear to be evolutionary unrelated to those from green photosynthetic organisms.
AB - The cDNA of the gene pds from tomato, encoding the carotenoid biosynthesis enzyme phytoene desaturase, was cloned, and its nucleotide sequence was determined. Cells of Escherichia coli that expressed the tomato pds gene could convert phytoene to ξ-carotene. This result suggests that one polypeptide, the product of the pds gene, can carry out phytoene desaturation in the carotenoid biosynthetic pathway. Transcripts of the pds gene accumulate in orange tomato fruit, indicating transcriptional control of pds expression during fruit ripening. The deduced amino acid sequence of phytoene desaturase indicates that this enzyme in tomato contains 583 amino acids that are highly conserved with respect to the homologous enzymes in cyanobacteria and algae. The deduced amino acid sequences of the phytoene desaturases from other microorganisms (purple bacteria and fungi) appear to be evolutionary unrelated to those from green photosynthetic organisms.
KW - Carotenoid biosynthesis
KW - Phytoene desaturase
KW - Plastids
UR - http://www.scopus.com/inward/record.url?scp=0026653382&partnerID=8YFLogxK
U2 - 10.1073/pnas.89.11.4962
DO - 10.1073/pnas.89.11.4962
M3 - Article
AN - SCOPUS:0026653382
SN - 0027-8424
VL - 89
SP - 4962
EP - 4966
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 11
ER -