A Synchronized Increase of Stilbenes and Flavonoids in Metabolically Engineered Vitis vinifera cv. Gamay Red Cell Culture

Ru Wang, Sangram Keshari Lenka, Varun Kumar, Noga Sikron-Persi, Irena Dynkin, David Weiss, Avichai Perl, Aaron Fait, Michal Oren-Shamir

Research output: Contribution to journalArticlepeer-review

Abstract

Stilbenes and flavonoids are two major health-promoting phenylpropanoid groups in grapes. Attempts to promote the accumulation of one group usually resulted in a decrease in the other. This study presents a unique strategy for simultaneously increasing metabolites in both groups in V. vinifera cv. Gamay Red grape cell culture, by overexpression of flavonol synthase (FLS) and increasing Phe availability. Increased Phe availability was achieved by transforming the cell culture with a second gene, the feedback-insensitive E. coli DAHP synthase (AroG*), and feeding them with Phe. A combined metabolomic and transcriptomic analysis reveals that the increase in both phenylpropanoid groups is accompanied by an induction of many of the flavonoid biosynthetic genes and no change in the expression levels of stilbene synthase. Furthermore, FLS overexpression with increased Phe availability resulted in higher anthocyanin levels, mainly those derived from delphinidin, due to the induction of F3′5′H. These insights may contribute to the development of grape berries with increased health benefits.

Original languageEnglish
Pages (from-to)7922-7931
Number of pages10
JournalJournal of Agricultural and Food Chemistry
Volume69
Issue number28
DOIs
StatePublished - 21 Jul 2021

Keywords

  • 3-deoxy- d -arabino-heptulosonate 7-phosphate synthase
  • Vitis vinifera
  • flavonol synthase
  • metabolic engineering
  • phenylalanine
  • precursor feeding
  • stilbene and flavonoid pathway

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