A synthetic peptide with estrogen-like activity derived from a phage-display peptide library

Natarajan Venkatesh, Yehudith Zaltsman, Dalia Somjen, Batya Gayer, Ettickan Boopathi, Roni Kasher, Tikva Kulik, Ephraim Katchalski-Katzir, Fortune Kohen

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

We describe a novel approach to develop peptides with estrogen like activity using a monoclonal antibody specific to estradiol (mAb E2-15) for the affinity selection of phage displayed peptides from a combinatorial peptide library. Based on the sequences of the selected phage, we synthesized a 15-mer linear peptide LPALDPTKRWFFETK which was derivatized to a 23 mer cyclic peptide CAELPALDPTKRWFFETKPPPPC. Both peptides displayed estrogen-like activity according to the following criteria:(i) in inhibiting the binding of [3H]estradiol to mAb E2-15 and to estrogen receptor (ER)α (ii) in inducing transcriptional activity in MCF7 human breast cancer cells transfected with an estrogen receptor element luciferase construct and (iii) in causing an increase in creatine kinase specific activity in rat tissues in vivo. This approach can be employed to design peptide mimetic for other hormones as well.

Original languageEnglish
Pages (from-to)573-580
Number of pages8
JournalPeptides
Volume23
Issue number3
DOIs
StatePublished - 23 Feb 2002
Externally publishedYes

Keywords

  • Estrogen receptor α
  • Estrogen receptor β
  • Monoclonal anti-estradiol antibody
  • Phage-display peptide library
  • Steroid-mimetic peptides

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