A temperature-sensitive mutation constructed by "linker insertion" mutagenesis

John Colicelli, Leslie I. Lobel, Stephen P. Goff

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


The in vitro mutagenesis of cloned DNAs allows the formation of virtually any specific mutation, but no method has been found which might routinely lead to the important phenotype of temperature sensitivity. We have studied three linker insertion mutations in the envelope gene of Moloney murine leukemia virus (M-MuLV), and found that one was exquisitely temperature-sensitive for plaque formation. We suggest that the construction of short insertion mutations may be a fruitful approach for the generation of temperature-sensitive phenotypes in cloned genes.

Original languageEnglish
Pages (from-to)537-539
Number of pages3
JournalMolecular Genetics and Genomics
Issue number3
StatePublished - 1 Jun 1985
Externally publishedYes

ASJC Scopus subject areas

  • Genetics


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