A toolbox and procedural notes for characterizing novel zinc finger nucleases for genome editing in plant cells

Keyphrases

• Plant Cells 100%
• Procedural 100%
• Genome Editing 100%
• Zinc Finger nuclease 100%
• Double-strand Breaks 18%
• Gene Targeting 18%
• Helix 9%
• Triplet 9%
• Palindromic 9%
• Activity Assay 9%
• Custom Design 9%
• Cassette 9%
• Efficient Implementation 9%
• Homologous Recombination 9%
• Oligonucleotides 9%
• De-novo Assembly 9%
• Restriction Enzyme 9%
• Expression Vector 9%
• Specific DNA Sequence 9%
• Nucleic Acids 9%
• DNA Binding 9%
• Targeted Sequencing 9%
• Agrobacterium Tumefaciens 9%
• Site-directed mutagenesis 9%
• Binding Region 9%
• Endonuclease 9%
• Plant Genome 9%
• DNA-binding Domain 9%
• Binary Plasmids 9%
• GUS Expression 9%
• FokI 9%
• Expression Cassette 9%
• Plant Expression 9%
• Plant Genes 9%
• UidA 9%
• Escherichia Coli Expression 9%
• Acid Digestion 9%
• Confirmatory Assay 9%
• In Vitro Digestion 9%
• Bacterial Lysates 9%

Biochemistry, Genetics and Molecular Biology

• Genome Editing 100%
• Zinc Finger Nuclease 100%
• Plant 27%
• Gene Targeting 18%
• Plasmid 9%
• DNA Sequence 9%
• Oligonucleotide 9%
• Homologous Recombination 9%
• Escherichia coli 9%
• Restriction Enzyme 9%
• Expression Vector 9%
• Nucleic Acid 9%
• Reconstruction 9%
• Cell Lysate 9%
• Agrobacterium tumefaciens 9%
• DNA Binding 9%
• Site-Directed Mutagenesis 9%
• Endonuclease 9%
• Plant Genome 9%
• DNA-binding Domain 9%
• FokI 9%
• Expression Cassette 9%
• Plant Gene 9%