@article{128952fc27f8424da225a339320c8794,
title = "A transplantable mouse tumor deficient in cyclic-AMP-dependent protein kinase activity",
abstract = "A transplantable solid tumor deficient in cyclic-AMP-dependent protein kinase (PK) (ATP: protein phosphotransferase; E.C.2.7.1.37) activity was established in vivo following injection into recipient nude mice of mutant (PK-)S49 mouse lymphoma cells propagated in vitro in suspension culture. Tumor cell growth was not affected by dibutyryl cyclic AMP (DB-cAMP) when transferred back into culture. These results suggest that cyclic AMP (cAMP) is not essential for cellular growth both in vitro and in vivo. In addition it is suggested that the absence of cAMP dependent protein kinase is related to the tumorigenicity of the cells by rendering them less controlable by environmental stimuli in the host.",
author = "Jacob Hochman and Aviva Katz and Yacob Weinstein",
note = "Funding Information: CYCLm-AMP is known to affect eukaryotic cells propagated in culture in a multitude of ways among which are cellular differentiation, cell cycle related phenomena and malignant transformation [1]. C,y clic-AMP causes growth arrest followed by cell death 48-72 hr after its administration to $49 mouse lymphoma cells in culture [2]. On the basis of this finding, it has been possible to select for, isolate and describe various mutants [3]. These have been partially characterized to the extent that their biological response to the drug could be directly related to specific lesions in either the membrane bound adenyl cyclase or the cyclic-AMP-dependent protein kinase or to a lesion distal to the protein kinase [4-7]. Cyclic-AMP has also been shown to cause growth arrest at the G-1 phase of the cell cycle. In mutant $49 cells lacking cyclic-AMP-dependent protein kinase activity, cells maintained a normal cell cycle in culture both in the absence: and presence of cyclic AMP [81 . It may be argued that the kind of inter- *This work was supported by grants fi'o,n tile Israel Commission for Basic Research, and the United States-Israel Binational Science Foundation (no.--1470) to J.H. .+To whom correspondence should be addressed.",
year = "1979",
month = jan,
day = "1",
doi = "10.1016/0014-2964(79)90199-3",
language = "English",
volume = "15",
pages = "11--13,15--16",
journal = "European Journal of Cancer (1965)",
issn = "0014-2964",
publisher = "Pergamon Press Ltd.",
number = "1",
}
