A wide-range, low-cost 150 bp ladder for sizing DNA fragments between 150 and 4500 bp

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7 Scopus citations

Abstract

Agarose gel electrophoresis, a very routine procedure, requires molecular weight standards; these are usually manufactured from plasmid or viral DNA fragments, or more recently, from PCR products of defined sizes. We describe here the preparation of a molecular weight standard from a completely different DNA source - the uniquely organized genome of the beetle Tenebrio molitor. The standard can be used to accurately size DNAs between 150 and 4500 bp, a useful range of sizes for many agarose gel electrophoresis applications, including separation of PCR products and plasmid cloning targets. In addition, it is easy to prepare, inexpensive, and rivals the best of the commercial ladders.

Original languageEnglish
Pages (from-to)900-902
Number of pages3
JournalElectrophoresis
Volume28
Issue number6
DOIs
StatePublished - 1 Jan 2007

Keywords

  • Molecular weight standard
  • Nucleic acid
  • Satellite DNA
  • Size marker

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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