TY - UNPB
T1 - Accelerated protein engineering using Vibrio natriegens genetic code expansion
AU - Ozer, Eden
AU - Alfonta, Lital
PY - 2020/7/7
Y1 - 2020/7/7
N2 - Escherichia coli has been considered as the most used model bacteria in the majority of studies for several decades. However, a new faster chassis is emerging in the form of the fast-growing gram-negative bacterium Vibrio natriegens. Different methodologies, well established in E. coli, are currently being adapted for the Vibrio natriegens in the hope of enabling a much faster platform for general lab-work. Amongst the vast technologies available for E. coli, genetic code expansion, the incorporation of unnatural amino acids into proteins, serves as a robust tool for protein engineering and biorthogonal modifications. Here we designed and adapted the genetic code expansion methodology for Vibrio natriegens and demonstrate an unnatural amino acid incorporation into a protein for the first time in this organism.
AB - Escherichia coli has been considered as the most used model bacteria in the majority of studies for several decades. However, a new faster chassis is emerging in the form of the fast-growing gram-negative bacterium Vibrio natriegens. Different methodologies, well established in E. coli, are currently being adapted for the Vibrio natriegens in the hope of enabling a much faster platform for general lab-work. Amongst the vast technologies available for E. coli, genetic code expansion, the incorporation of unnatural amino acids into proteins, serves as a robust tool for protein engineering and biorthogonal modifications. Here we designed and adapted the genetic code expansion methodology for Vibrio natriegens and demonstrate an unnatural amino acid incorporation into a protein for the first time in this organism.
U2 - 10.22541/au.159414764.44184665
DO - 10.22541/au.159414764.44184665
M3 - Preprint
BT - Accelerated protein engineering using Vibrio natriegens genetic code expansion
ER -