Activation of mitogen-activated protein kinases in human heart during cardiopulmonary bypass

Daniel Talmor, Azai Applebaum, Assaf Rudich, Yoram Shapira, Amir Tirosh

Research output: Contribution to journalArticlepeer-review

32 Scopus citations


Mitogen-activated protein kinases (MAPKs) have been shown to be activated in both in vitro and in vivo models of cardiac tissue in response to ischemia/reperfusion injury. We investigated whether MAPKs are activated in human heart during coronary artery bypass grafting (CABG) surgery. During elective CABG surgery of 8 patients, 3 fight atrial appendage biopsies were obtained at baseline, at the end of cross-clamping, and after coronary reperfusion. The expression of the p38-MAPK, c-Jun N-terminal kinase (JNK), and extracellular signal-regulated kinases (ERK1/2) MAPKs was not altered during CABG. The phosphorylation and activation of both ERK1/2 and p38-MAPK were increased ≃2-fold by ischemia and even more (8- and 4-fold, respectively) by reperfusion. Although the ischemic period did not result in a significant activation of JNK, an ≃6-fold increase in JNK activity could be observed after reperfusion. In conclusion, distinct activation patterns of ERK1/2, p38, and JNK MAPKs can be observed in human heart during CABG.

Original languageEnglish
Pages (from-to)1004-1007
Number of pages4
JournalCirculation Research
Issue number9
StatePublished - 12 May 2000


  • Ischemia/reperfusion
  • Myocardium
  • Stress-activated protein kinases

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine


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