Active O-acetylserine-(thiol) lyase A and B confer improved selenium resistance and degrade l-Cys and l-SeCys in Arabidopsis

Assylay Kurmanbayeva, Aizat Bekturova, Aigerim Soltabayeva, Dinara Oshanova, Zhadyrassyn Nurbekova, Sudhakar Srivastava, Poonam Tiwari, Arvind Kumar Dubey, Moshe Sagi

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The roles of cytosolic O-acetylserine-(thiol)-lyase A (OASTLA), chloroplastic OASTLB, and mitochondrial OASTLC in plant selenate resistance were studied in Arabidopsis. Impairment in OASTLA and OASTLB resulted in reduced biomass, chlorophyll and soluble protein content compared with selenate-treated OASTLC-impaired and wild-type plants. The generally lower total selenium (Se), protein-Se, organic-sulfur and protein-sulfur (S) content in oastlA and oastlB compared with wild-type and oastlC leaves indicated that Se accumulation was not the main cause for the stress symptoms in these mutants. Notably, the application of selenate positively induced S-starvation markers and the OASTLs, followed by increased sulfite reductase, sulfite oxidase activities, and increased sulfite and sulfide concentrations. Taken together, our results indicate a futile anabolic S-starvation response that resulted in lower glutathione and increased oxidative stress symptoms in oastlA and oastlB mutants. In-gel assays of l-cysteine and l-seleno-cysteine, desulfhydrase activities revealed that two of the three OASTL activity bands in each of the oastl single mutants were enhanced in response to selenate, whereas the impaired proteins exhibited a missing activity band. The absence of differently migrated activity bands in each of the three oastl mutants indicates that these OASTLs are major components of desulfhydrase activity, degrading l-cysteine and l-seleno-cysteine in Arabidopsis.

Original languageEnglish
Pages (from-to)2525-2539
Number of pages15
JournalJournal of Experimental Botany
Volume73
Issue number8
DOIs
StatePublished - 18 Apr 2022

Keywords

  • Arabidopsis
  • L-cysteine desulfhydrase
  • L-seleno-cysteine desulfhydrase
  • OASTLs
  • selenium metabolism
  • sulfur metabolism

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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