Adduct of fullerene C60 with l-arginine: interaction with DNA, genotoxic and mutagenic properties

Olegi N. Kukaliia; Sergei V. Ageev; Andrey V. Petrov; Olga S. Shemchuk; Elena V. Sambuk; Andrey M. Rumyantsev; Daler R. Dadadzhanov; Marina A. Korzhenevskaia; Oleg E. Molchanov; Dmitrii N. Maistrenko; Igor V. Murin; Vladimir V. Sharoyko; Konstantin N. Semenov

doi:10.1080/1536383X.2024.2344768

Adduct of fullerene C₆₀ with l-arginine: interaction with DNA, genotoxic and mutagenic properties

Olegi N. Kukaliia, Sergei V. Ageev, Andrey V. Petrov, Olga S. Shemchuk, Elena V. Sambuk, Andrey M. Rumyantsev, Daler R. Dadadzhanov, Marina A. Korzhenevskaia, Oleg E. Molchanov, Dmitrii N. Maistrenko, Igor V. Murin, Vladimir V. Sharoyko, Konstantin N. Semenov

Research output: Contribution to journal › Article › peer-review

Abstract

The paper presents a physicochemical study of the water-soluble adduct of C₆₀ with l-arginine, including a quantum chemical determination of the stability of isomers, measurement of antiradical activity towards the ABTS radical, determination of the lipophilicity coefficient, particle size distribution in aqueous solutions and calculation of ζ-potentials. A separate part of the article is devoted to the study of the effect of the C₆₀ adduct with l-arginine on DNA in in vitro experiments (determination of the binding constant using isothermal titration calorimetry, study of genotoxicity, DNA melting temperature, effect on DNA circular dichroism spectra). Data are also presented on the biodistribution of the C₆₀ adduct with l-arginine in the organs and tissues of animals and its effect on clinical and biochemical blood parameters. The mutagenic effect of the C₆₀ adduct with l-arginine was determined in vivo on a model of mice abnormal sperm heads. It was shown that the adduct is in a neutral state in physiological solution, has an affinity to both aqueous and lipid phases (logP_ow = 0.23), and forms a complex with DNA (K_a = 4.59 10⁶ M ⁻¹), while it does not have cyto- and genotoxicity in vitro and mutagenicity in vivo.

Original language	English
Journal	Fullerenes Nanotubes and Carbon Nanostructures
DOIs	https://doi.org/10.1080/1536383X.2024.2344768
State	Accepted/In press - 1 Jan 2024
Externally published	Yes

Keywords

biodistribution
cytotoxicity
Fullerene
genotoxicity
mutagenicity

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
General Materials Science
Physical and Theoretical Chemistry
Organic Chemistry

Access to Document

10.1080/1536383X.2024.2344768

Cite this

Kukaliia, O. N., Ageev, S. V., Petrov, A. V., Shemchuk, O. S., Sambuk, E. V., Rumyantsev, A. M., Dadadzhanov, D. R., Korzhenevskaia, M. A., Molchanov, O. E., Maistrenko, D. N., Murin, I. V., Sharoyko, V. V., & Semenov, K. N. (Accepted/In press). Adduct of fullerene C₆₀ with l-arginine: interaction with DNA, genotoxic and mutagenic properties. Fullerenes Nanotubes and Carbon Nanostructures. https://doi.org/10.1080/1536383X.2024.2344768

@article{7212d7f3be1b407c974cffddf604add3,

title = "Adduct of fullerene C60 with l-arginine: interaction with DNA, genotoxic and mutagenic properties",

abstract = "The paper presents a physicochemical study of the water-soluble adduct of C60 with l-arginine, including a quantum chemical determination of the stability of isomers, measurement of antiradical activity towards the ABTS radical, determination of the lipophilicity coefficient, particle size distribution in aqueous solutions and calculation of ζ-potentials. A separate part of the article is devoted to the study of the effect of the C60 adduct with l-arginine on DNA in in vitro experiments (determination of the binding constant using isothermal titration calorimetry, study of genotoxicity, DNA melting temperature, effect on DNA circular dichroism spectra). Data are also presented on the biodistribution of the C60 adduct with l-arginine in the organs and tissues of animals and its effect on clinical and biochemical blood parameters. The mutagenic effect of the C60 adduct with l-arginine was determined in vivo on a model of mice abnormal sperm heads. It was shown that the adduct is in a neutral state in physiological solution, has an affinity to both aqueous and lipid phases (logPow = 0.23), and forms a complex with DNA (Ka = 4.59 106 M −1), while it does not have cyto- and genotoxicity in vitro and mutagenicity in vivo.",

keywords = "biodistribution, cytotoxicity, Fullerene, genotoxicity, mutagenicity",

author = "Kukaliia, {Olegi N.} and Ageev, {Sergei V.} and Petrov, {Andrey V.} and Shemchuk, {Olga S.} and Sambuk, {Elena V.} and Rumyantsev, {Andrey M.} and Dadadzhanov, {Daler R.} and Korzhenevskaia, {Marina A.} and Molchanov, {Oleg E.} and Maistrenko, {Dmitrii N.} and Murin, {Igor V.} and Sharoyko, {Vladimir V.} and Semenov, {Konstantin N.}",

note = "Publisher Copyright: {\textcopyright} 2024 Taylor & Francis Group, LLC.",

year = "2024",

month = jan,

day = "1",

doi = "10.1080/1536383X.2024.2344768",

language = "English",

journal = "Fullerenes Nanotubes and Carbon Nanostructures",

issn = "1536-383X",

publisher = "Taylor and Francis Ltd.",

}

Kukaliia, ON, Ageev, SV, Petrov, AV, Shemchuk, OS, Sambuk, EV, Rumyantsev, AM, Dadadzhanov, DR, Korzhenevskaia, MA, Molchanov, OE, Maistrenko, DN, Murin, IV, Sharoyko, VV & Semenov, KN 2024, 'Adduct of fullerene C₆₀ with l-arginine: interaction with DNA, genotoxic and mutagenic properties', Fullerenes Nanotubes and Carbon Nanostructures. https://doi.org/10.1080/1536383X.2024.2344768

TY - JOUR

T1 - Adduct of fullerene C60 with l-arginine

T2 - interaction with DNA, genotoxic and mutagenic properties

AU - Kukaliia, Olegi N.

AU - Ageev, Sergei V.

AU - Petrov, Andrey V.

AU - Shemchuk, Olga S.

AU - Sambuk, Elena V.

AU - Rumyantsev, Andrey M.

AU - Dadadzhanov, Daler R.

AU - Korzhenevskaia, Marina A.

AU - Molchanov, Oleg E.

AU - Maistrenko, Dmitrii N.

AU - Murin, Igor V.

AU - Sharoyko, Vladimir V.

AU - Semenov, Konstantin N.

PY - 2024/1/1

Y1 - 2024/1/1

N2 - The paper presents a physicochemical study of the water-soluble adduct of C60 with l-arginine, including a quantum chemical determination of the stability of isomers, measurement of antiradical activity towards the ABTS radical, determination of the lipophilicity coefficient, particle size distribution in aqueous solutions and calculation of ζ-potentials. A separate part of the article is devoted to the study of the effect of the C60 adduct with l-arginine on DNA in in vitro experiments (determination of the binding constant using isothermal titration calorimetry, study of genotoxicity, DNA melting temperature, effect on DNA circular dichroism spectra). Data are also presented on the biodistribution of the C60 adduct with l-arginine in the organs and tissues of animals and its effect on clinical and biochemical blood parameters. The mutagenic effect of the C60 adduct with l-arginine was determined in vivo on a model of mice abnormal sperm heads. It was shown that the adduct is in a neutral state in physiological solution, has an affinity to both aqueous and lipid phases (logPow = 0.23), and forms a complex with DNA (Ka = 4.59 106 M −1), while it does not have cyto- and genotoxicity in vitro and mutagenicity in vivo.

AB - The paper presents a physicochemical study of the water-soluble adduct of C60 with l-arginine, including a quantum chemical determination of the stability of isomers, measurement of antiradical activity towards the ABTS radical, determination of the lipophilicity coefficient, particle size distribution in aqueous solutions and calculation of ζ-potentials. A separate part of the article is devoted to the study of the effect of the C60 adduct with l-arginine on DNA in in vitro experiments (determination of the binding constant using isothermal titration calorimetry, study of genotoxicity, DNA melting temperature, effect on DNA circular dichroism spectra). Data are also presented on the biodistribution of the C60 adduct with l-arginine in the organs and tissues of animals and its effect on clinical and biochemical blood parameters. The mutagenic effect of the C60 adduct with l-arginine was determined in vivo on a model of mice abnormal sperm heads. It was shown that the adduct is in a neutral state in physiological solution, has an affinity to both aqueous and lipid phases (logPow = 0.23), and forms a complex with DNA (Ka = 4.59 106 M −1), while it does not have cyto- and genotoxicity in vitro and mutagenicity in vivo.

KW - biodistribution

KW - cytotoxicity

KW - Fullerene

KW - genotoxicity

KW - mutagenicity

UR - http://www.scopus.com/inward/record.url?scp=85192243002&partnerID=8YFLogxK

U2 - 10.1080/1536383X.2024.2344768

DO - 10.1080/1536383X.2024.2344768

M3 - Article

AN - SCOPUS:85192243002

SN - 1536-383X

JO - Fullerenes Nanotubes and Carbon Nanostructures

JF - Fullerenes Nanotubes and Carbon Nanostructures

ER -