TY - JOUR
T1 - Adenovirus expressing a bioluminescence reporter gene and cMAGI cell assay for the detection of HIV-1
AU - Borkow, Gadi
AU - Lara, Humberto Herman
AU - Ayash-Rashkovsky, Mila
AU - Tavor, Einat
AU - Lapidot, Aviva
AU - Bentwich, Zvi
AU - Honigman, Alik
N1 - Funding Information:
The work was supported by grants from the Horowitz Foundation (to Kaplan AIDS Center) and Tmura, Ministry of Commerce and Industry, Israel (to AH).
PY - 2004/10/1
Y1 - 2004/10/1
N2 - We report a fast, highly sensitive method for detecting and testing drug resistance of M-tropic and T-tropic laboratory and primary HIV-1 isolates. cMAGI cells are infected with an adenovirus vector harboring the luciferase reporter gene controlled by HIV-1 Tat-responsive element, TAR. HIV-1 Tat production by HIV-1 chronically infected cells, or by cMAGI cells as early as two days after being acutely infected with HIV-1, is readily monitored in the presence or absence of antiviral drugs. This method is more sensitive than HIV-1 Tat dependant production of β-galactosidase in the cMAGI cells. The fast answer, ease and sensitivity as well as the possibility of using this method in high throughput screening, makes it an very attractive tool for phenotypic detection of HIV-1 in clinical samples as well as a sensitive assay for monitoring drug resistant HIV-1 variants. This method can also be used for discovery of novel anti HIV-1 drugs.
AB - We report a fast, highly sensitive method for detecting and testing drug resistance of M-tropic and T-tropic laboratory and primary HIV-1 isolates. cMAGI cells are infected with an adenovirus vector harboring the luciferase reporter gene controlled by HIV-1 Tat-responsive element, TAR. HIV-1 Tat production by HIV-1 chronically infected cells, or by cMAGI cells as early as two days after being acutely infected with HIV-1, is readily monitored in the presence or absence of antiviral drugs. This method is more sensitive than HIV-1 Tat dependant production of β-galactosidase in the cMAGI cells. The fast answer, ease and sensitivity as well as the possibility of using this method in high throughput screening, makes it an very attractive tool for phenotypic detection of HIV-1 in clinical samples as well as a sensitive assay for monitoring drug resistant HIV-1 variants. This method can also be used for discovery of novel anti HIV-1 drugs.
KW - HIV-1
KW - TAR
KW - adenovirus
KW - cMAGI
KW - luciferase
KW - reporter gene
UR - http://www.scopus.com/inward/record.url?scp=4544226072&partnerID=8YFLogxK
U2 - 10.1023/B:VIRU.0000036386.47149.62
DO - 10.1023/B:VIRU.0000036386.47149.62
M3 - Article
C2 - 15284486
AN - SCOPUS:4544226072
SN - 0920-8569
VL - 29
SP - 257
EP - 265
JO - Virus Genes
JF - Virus Genes
IS - 2
ER -
