Adhesion of human platelets to serum amyloid A

Simcha Urieli-Shoval, George Shubinsky, Reinhold P. Linke, Mati Fridkin, Israel Tabi, Yaacov Matzner

Research output: Contribution to journalArticlepeer-review

64 Scopus citations


Serum amyloid A (SAA) is an acute phase reactant, and its level in the blood is elevated to 1000-fold in response of the body to trauma, infection, inflammation, and neoplasia. SAA was reported to inhibit platelet aggregation and to induce adhesion of leukocytes. This study looked at adhesion of human platelets to SAA. Immobilized SAA supported the adhesion of human washed platelets; level of adhesion to SAA was comparable to fibronectin and lower than to fibrinogen. Adhesion to SAA was further enhanced by Mn2+ and the physiological agonist, thrombin. Platelet adhesion to SAA was completely abolished by anti-SAA antibody. SAA-induced adhesion was inhibited by antibodies against the integrin receptor αIIbβ3, by the peptide GRGDSP and by SAA-derived peptide containing YIGSR-like and RGD-like adhesion motifs (amino acids 29 to 42). Adhesion was not inhibited by control immunoglobulin G, by antibody against the integrin receptor αVβ3, by the peptide GRGESP, and by SAA-derived peptide that includes incomplete RGD motif. SAA-derived peptide 29 to 42 also inhibited platelet adhesion to fibronectin. Transfected human melanoma cells expressing αIIbβ3 adhered to SAA, whereas transfected cells expressing αVβ3 did not. By using flow cytometry, the αIIbβ3 cells displayed significantly higher levels of binding of soluble SAA than the αVβ3 cells. These data indicate that human platelets specifically adhere to SAA in an RGD- and αIIbβ3-dependent manner. Thus, SAA may play a role in modulating platelet adhesion at vascular injury sites by sharing platelet receptors with other platelet-adhesive proteins.

Original languageEnglish
Pages (from-to)1224-1229
Number of pages6
Issue number4
StatePublished - 15 Feb 2002
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology


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