Advanced cloning tools for construction of designer cellulosomes

Amaranta Kahn, Edward A. Bayer, Sarah Moraïs

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

7 Scopus citations


Cellulose deconstruction is achieved in nature through two main enzymatic paradigms, i.e., free enzymes and enzymatic complexes (called cellulosomes). Gaining insights into the mechanism of action and synergy among the different cellulases is of high interest, notably in the field of renewable energy, and specifically, for the conversion of cellulosic biomass to soluble sugars, en route to biofuels. In this context, designer cellulosomes are artificially assembled, chimaeric protein complexes that are used as a tool to comparatively study cellulose degradation by different enzymatic paradigms, and could also serve to improve cellulose deconstruction. Various molecular biology techniques are employed in order to design and engineer the various components of designer cellulosomes. In this chapter, we describe the cloning processes through which the appropriate modules are selected and assembled at the molecular level.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages17
StatePublished - 1 Jan 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • CPEC
  • Chimaeric enzyme
  • Cloning
  • Cohesin
  • Dockerin
  • Enzymatic complex
  • Scaffoldin

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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