Aflp markers for sex determination in an ilex species; Marqueurs aflp pour la determination du sexe chez une espece d'ilex

A. Golan-Goldhirsh, R. Jones, L. J. Rowland

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    4 Scopus citations

    Abstract

    Dioecious Ilex species (holly) represent a unique plant model to search for sex related molecular markers. Little is known about the genetic and physiological basis for sex determination in this genus and modern molecular marker techniques may provide tools for studying its mechanism, as well as assist in breeding and plant propagation programs to identify the female berry-producing sex at the seedling stage. In this study, female I. crenata Sky Pencil cv., male I. crenata Microphylla cv. and their F1 progeny were analyzed for the presence of AFLP markers associated with sex determination. We present here the results from a bulked-segregant analysis (BSA) using AFLP markers. Seven AFLP markers which were identified as being associated with either the male or female sex, were isolated from the gels, cloned and sequenced.

    Original languageEnglish
    Title of host publicationInternational Symposium on Molecular Markers for Characterizing Genotypes and Identifying Cultivars in Horticulture
    Editors C. Dore, F. Dosba, C. Baril
    Pages591-595
    Number of pages5
    StatePublished - 1 Dec 2001

    Publication series

    NameActa Horticulturae
    Volume546
    ISSN (Print)0567-7572

    Keywords

    • Cloning
    • Dioecy
    • Holly
    • Ilex crenata
    • Molecular markers
    • PCR

    ASJC Scopus subject areas

    • Horticulture

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