Age related changes in hemopoietic capacity of bone marrow cells

A. Sharp, D. Zipori, J. Toledo, S. Tal, P. Resnitzky, A. Globerson

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


The effect of aging on the hemopoietic capacity of bone marrow (BM) cells was investigated. No difference was found in the incidence of colony forming units-spleen (CFU-S) and granulocyte-macrophage colony forming cells (GM-CFC) present in the BM of young (2-4 months) or old (24-30 months) mice. However, increased proliferations (× 3) of the old BM cells was observed when cultured in the presence of L-cell conditioned medium. The cells were also cultivated over an adherent layer of a BM stroma cell line (14F1.1, endothelial-adipocytes) and the non-adherent cells removed and tested weekly. Cells orginating from the old BM proliferated to a greater extent and produced more GM-CFC than those from the young. Differentiation into T-cells after colonizing fetal thymus explants was also measured and found to be reduced in both groups, though to a greater extent in the old. Thus, under the present experimental conditions, myeloid progenitors in the old BM manifest a more pronounced self renewal and differentiation capacity than the young while for the T-cell pregenitors the situation is reversed.

Original languageEnglish
Pages (from-to)91-99
Number of pages9
JournalMechanisms of Ageing and Development
Issue number1
StatePublished - 1 Jan 1989
Externally publishedYes


  • Aging
  • Bone marrow
  • CFU-S
  • GM-CFC

ASJC Scopus subject areas

  • Aging
  • Developmental Biology


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