TY - JOUR
T1 - Agrobacterium-mediated genetic transformation of plants
T2 - biology and biotechnology
AU - Tzfira, Tzvi
AU - Citovsky, Vitaly
N1 - Funding Information:
We apologize to colleagues whose original works were omitted owing to space constraints. The work in our laboratories was supported by grants from the Human Frontiers Science Program (HFSP) and the US-Israel Bi-National Agricultural Research and Development Fund (BARD) to TT, and from the National Institutes of Health, National Science Foundation, US Department of Agriculture, US-Israel Science Foundation (BSF), and BARD to VC.
PY - 2006/4/1
Y1 - 2006/4/1
N2 - Agrobacterium-mediated genetic transformation is the dominant technology used for the production of genetically modified transgenic plants. Extensive research aimed at understanding and improving the molecular machinery of Agrobacterium responsible for the generation and transport of the bacterial DNA into the host cell has resulted in the establishment of many recombinant Agrobacterium strains, plasmids and technologies currently used for the successful transformation of numerous plant species. Unlike the role of bacterial proteins, the role of host factors in the transformation process has remained obscure for nearly a century of Agrobacterium research, and only recently have we begun to understand how Agrobacterium hijacks host factors and cellular processes during the transformation process. The identification of such factors and studies of these processes hold great promise for the future of plant biotechnology and plant genetic engineering, as they might help in the development of conceptually new techniques and approaches needed today to expand the host range of Agrobacterium and to control the transformation process and its outcome during the production of transgenic plants.
AB - Agrobacterium-mediated genetic transformation is the dominant technology used for the production of genetically modified transgenic plants. Extensive research aimed at understanding and improving the molecular machinery of Agrobacterium responsible for the generation and transport of the bacterial DNA into the host cell has resulted in the establishment of many recombinant Agrobacterium strains, plasmids and technologies currently used for the successful transformation of numerous plant species. Unlike the role of bacterial proteins, the role of host factors in the transformation process has remained obscure for nearly a century of Agrobacterium research, and only recently have we begun to understand how Agrobacterium hijacks host factors and cellular processes during the transformation process. The identification of such factors and studies of these processes hold great promise for the future of plant biotechnology and plant genetic engineering, as they might help in the development of conceptually new techniques and approaches needed today to expand the host range of Agrobacterium and to control the transformation process and its outcome during the production of transgenic plants.
UR - http://www.scopus.com/inward/record.url?scp=33645945885&partnerID=8YFLogxK
U2 - 10.1016/j.copbio.2006.01.009
DO - 10.1016/j.copbio.2006.01.009
M3 - Review article
AN - SCOPUS:33645945885
SN - 0958-1669
VL - 17
SP - 147
EP - 154
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
IS - 2
ER -