Aldehyde oxidase in roots, leaves and seeds of barley (Hordeum vulgare L.)

Rustem T. Omarov, Shuichi Akaba, Tomokazu Koshiba, S. Herman Lips

    Research output: Contribution to journalArticlepeer-review

    37 Scopus citations

    Abstract

    Aldehyde oxidase (AO, EC 1.2.3.1) proteins in leaves, roots and seeds of barley (Hordeum vulgare L.) plants were studied. Differences in substrate specificity and mobility in native PAGE between AO proteins extracted from roots, leaves and seeds have been observed. Four clear bands of AO reacting proteins were detected in barley plants capable of oxidizing a number of aliphatic and aromatic aldehydes such as indole-3-aldehyde, acetaldehyde, heptaldehyde, and benzaldehyde. Mouse polyclonal antibodies raised against purified maize AO cross-reacted with barley AO proteins extracted from roots, leaves and seeds. At least three different AO proteins were detected in roots on the basis of their mobility during PAGE after native Western blot analysis while in leaves and seeds only one polypeptide cross-reacted with the antibody. SDS-immunoblot analysis showed marked differences in molecular weight between subunits of the AO bands extracted from roots, leaves and seeds. Two distinct subunit bands were observed in roots, with relatively close molecular weights (160 kDa and 145 kDa), while a single subunit with a molecular weight of 150 kDa was observed in leaf and seed extracts. Menadione, a specific and potent inhibitor of animal AO did not affect barley AO proteins. Root and leaf AO differed in their thermostability and susceptibility to exogenous tungstate. The AO proteins in plants may be a group of enzymes with different substrate specificity, tissue distribution and presumably fulfilling different metabolic roles in each plant organ.

    Original languageEnglish
    Pages (from-to)63-69
    Number of pages7
    JournalJournal of Experimental Botany
    Volume50
    Issue number330
    DOIs
    StatePublished - 1 Jan 1999

    Keywords

    • Aldehyde oxidase
    • Barley
    • Leaves
    • Molybdenum cofactor (MoCo)
    • Roots
    • Seeds

    ASJC Scopus subject areas

    • Physiology
    • Plant Science

    Fingerprint

    Dive into the research topics of 'Aldehyde oxidase in roots, leaves and seeds of barley (Hordeum vulgare L.)'. Together they form a unique fingerprint.

    Cite this