Aligning the μs-ALEX setup

Achillefs Kapanidis, Devdoot Majumdar, Mike Heilemann, Eyal Nir, Shimon Weiss

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


To achieve single-molecule sensitivity and thus have the ability to detect single diffusing fluorophores, careful alignment of the microsecond-alternating laser excitation (μs-Alex) setup is crucial. The following protocol describes routine alignment for 2c-ALEX (532 nm/635 nm) with spectral windows G550–620R650–750.

Original languageEnglish
Pages (from-to)1027-1028
Number of pages2
JournalCold Spring Harbor Protocols
Issue number11
StatePublished - 1 Nov 2015

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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