Aligning the μs-ALEX setup

Achillefs Kapanidis; Devdoot Majumdar; Mike Heilemann; Eyal Nir; Shimon Weiss

doi:10.1101/pdb.prot086421

Aligning the μs-ALEX setup

Achillefs Kapanidis, Devdoot Majumdar, Mike Heilemann, Eyal Nir, Shimon Weiss

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

To achieve single-molecule sensitivity and thus have the ability to detect single diffusing fluorophores, careful alignment of the microsecond-alternating laser excitation (μs-Alex) setup is crucial. The following protocol describes routine alignment for 2c-ALEX (532 nm/635 nm) with spectral windows G^550–620R^650–750.

Original language	English
Pages (from-to)	1027-1028
Number of pages	2
Journal	Cold Spring Harbor Protocols
Volume	2015
Issue number	11
DOIs	https://doi.org/10.1101/pdb.prot086421
State	Published - 1 Nov 2015

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1101/pdb.prot086421

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title = "Aligning the μs-ALEX setup",

abstract = "To achieve single-molecule sensitivity and thus have the ability to detect single diffusing fluorophores, careful alignment of the microsecond-alternating laser excitation (μs-Alex) setup is crucial. The following protocol describes routine alignment for 2c-ALEX (532 nm/635 nm) with spectral windows G550–620R650–750.",

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AU - Majumdar, Devdoot

AU - Heilemann, Mike

AU - Nir, Eyal

AU - Weiss, Shimon

PY - 2015/11/1

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N2 - To achieve single-molecule sensitivity and thus have the ability to detect single diffusing fluorophores, careful alignment of the microsecond-alternating laser excitation (μs-Alex) setup is crucial. The following protocol describes routine alignment for 2c-ALEX (532 nm/635 nm) with spectral windows G550–620R650–750.

AB - To achieve single-molecule sensitivity and thus have the ability to detect single diffusing fluorophores, careful alignment of the microsecond-alternating laser excitation (μs-Alex) setup is crucial. The following protocol describes routine alignment for 2c-ALEX (532 nm/635 nm) with spectral windows G550–620R650–750.

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Aligning the μs-ALEX setup

Abstract

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