Aligning the μs-ALEX setup

Achillefs Kapanidis; Devdoot Majumdar; Mike Heilemann; Eyal Nir; Shimon Weiss

doi:10.1101/pdb.prot086421

Aligning the μs-ALEX setup

Achillefs Kapanidis, Devdoot Majumdar, Mike Heilemann, Eyal Nir, Shimon Weiss

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

To achieve single-molecule sensitivity and thus have the ability to detect single diffusing fluorophores, careful alignment of the microsecond-alternating laser excitation (μs-Alex) setup is crucial. The following protocol describes routine alignment for 2c-ALEX (532 nm/635 nm) with spectral windows G^550–620R^650–750.

Original language	English
Pages (from-to)	1027-1028
Number of pages	2
Journal	Cold Spring Harbor Protocols
Volume	2015
Issue number	11
DOIs	https://doi.org/10.1101/pdb.prot086421
State	Published - 1 Nov 2015

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1101/pdb.prot086421

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title = "Aligning the μs-ALEX setup",

abstract = "To achieve single-molecule sensitivity and thus have the ability to detect single diffusing fluorophores, careful alignment of the microsecond-alternating laser excitation (μs-Alex) setup is crucial. The following protocol describes routine alignment for 2c-ALEX (532 nm/635 nm) with spectral windows G550–620R650–750.",

author = "Achillefs Kapanidis and Devdoot Majumdar and Mike Heilemann and Eyal Nir and Shimon Weiss",

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AU - Kapanidis, Achillefs

AU - Majumdar, Devdoot

AU - Heilemann, Mike

AU - Nir, Eyal

AU - Weiss, Shimon

PY - 2015/11/1

Y1 - 2015/11/1

N2 - To achieve single-molecule sensitivity and thus have the ability to detect single diffusing fluorophores, careful alignment of the microsecond-alternating laser excitation (μs-Alex) setup is crucial. The following protocol describes routine alignment for 2c-ALEX (532 nm/635 nm) with spectral windows G550–620R650–750.

AB - To achieve single-molecule sensitivity and thus have the ability to detect single diffusing fluorophores, careful alignment of the microsecond-alternating laser excitation (μs-Alex) setup is crucial. The following protocol describes routine alignment for 2c-ALEX (532 nm/635 nm) with spectral windows G550–620R650–750.

UR - https://www.scopus.com/pages/publications/84946040192

U2 - 10.1101/pdb.prot086421

DO - 10.1101/pdb.prot086421

M3 - Article

AN - SCOPUS:84946040192

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SP - 1027

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JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

IS - 11

ER -

Aligning the μs-ALEX setup

Abstract

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