All three J-domain proteins of the Escherichia coli DnaK chaperone machinery are DNA binding proteins

Eyal Gur; Chen Katz; Eliora Z. Ron

doi:10.1016/j.febslet.2005.01.084

All three J-domain proteins of the Escherichia coli DnaK chaperone machinery are DNA binding proteins

Eyal Gur, Chen Katz, Eliora Z. Ron

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

DnaJ, DjlA and CbpA are the J-domain proteins of DnaK, the major Hsp70 of Escherichia coli. CbpA was originally discovered as a DNA binding protein. Here, we show that DNA binding is a property of DnaJ and DjlA as well. Of special interest in this respect is DjlA, as this cytoplasmic protein is membrane bound and, as shown here, its affinity for DNA is extremely high. The finding that all the three J-proteins of DnaK are DNA binding proteins sheds new light on the cellular activity of these proteins.

Original language	English
Pages (from-to)	1935-1939
Number of pages	5
Journal	FEBS Letters
Volume	579
Issue number	9
DOIs	https://doi.org/10.1016/j.febslet.2005.01.084
State	Published - 28 Mar 2005
Externally published	Yes

Keywords

Chaperones
DNA binding proteins
Heat shock response

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2005.01.084

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title = "All three J-domain proteins of the Escherichia coli DnaK chaperone machinery are DNA binding proteins",

abstract = "DnaJ, DjlA and CbpA are the J-domain proteins of DnaK, the major Hsp70 of Escherichia coli. CbpA was originally discovered as a DNA binding protein. Here, we show that DNA binding is a property of DnaJ and DjlA as well. Of special interest in this respect is DjlA, as this cytoplasmic protein is membrane bound and, as shown here, its affinity for DNA is extremely high. The finding that all the three J-proteins of DnaK are DNA binding proteins sheds new light on the cellular activity of these proteins.",

keywords = "Chaperones, DNA binding proteins, Heat shock response",

author = "Eyal Gur and Chen Katz and Ron, {Eliora Z.}",

note = "Funding Information: We thank Ehud Gazit and Abdussalam Azem from the faculty of Life Sciences of Tel-Aviv University for their advice throughout this study. This research was supported, in part, by the Manja and Morris Leigh Chair for Biophysics and Biotechnology.",

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T1 - All three J-domain proteins of the Escherichia coli DnaK chaperone machinery are DNA binding proteins

AU - Gur, Eyal

AU - Katz, Chen

AU - Ron, Eliora Z.

N1 - Funding Information: We thank Ehud Gazit and Abdussalam Azem from the faculty of Life Sciences of Tel-Aviv University for their advice throughout this study. This research was supported, in part, by the Manja and Morris Leigh Chair for Biophysics and Biotechnology.

PY - 2005/3/28

Y1 - 2005/3/28

N2 - DnaJ, DjlA and CbpA are the J-domain proteins of DnaK, the major Hsp70 of Escherichia coli. CbpA was originally discovered as a DNA binding protein. Here, we show that DNA binding is a property of DnaJ and DjlA as well. Of special interest in this respect is DjlA, as this cytoplasmic protein is membrane bound and, as shown here, its affinity for DNA is extremely high. The finding that all the three J-proteins of DnaK are DNA binding proteins sheds new light on the cellular activity of these proteins.

AB - DnaJ, DjlA and CbpA are the J-domain proteins of DnaK, the major Hsp70 of Escherichia coli. CbpA was originally discovered as a DNA binding protein. Here, we show that DNA binding is a property of DnaJ and DjlA as well. Of special interest in this respect is DjlA, as this cytoplasmic protein is membrane bound and, as shown here, its affinity for DNA is extremely high. The finding that all the three J-proteins of DnaK are DNA binding proteins sheds new light on the cellular activity of these proteins.

KW - Chaperones

KW - DNA binding proteins

KW - Heat shock response

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DO - 10.1016/j.febslet.2005.01.084

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SN - 0014-5793

VL - 579

SP - 1935

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JO - FEBS Letters

JF - FEBS Letters

IS - 9

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All three J-domain proteins of the Escherichia coli DnaK chaperone machinery are DNA binding proteins

Abstract

Keywords

ASJC Scopus subject areas

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